Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Quantitative Analysis of Leuconostoc mesenteroides and Lactobacillus plantarum Populations by a Competitive Polymerase Chain Reaction

  • Koh, Young-Ho (School of Agricultural Biotechnology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • Kim, Myoung-Dong (School of Agricultural Biotechnology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • Han, Nam-Soo (Department of Food Science and Techology, Chungbuk National University) ;
  • Seo, Jin-Ho (School of Agricultural Biotechnology and Research Center for New Bio-Materials in Agriculture, Seoul National University)
  • Published : 2002.10.01
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Abstract

A multiplex competitive polymerase chain reaction (PCR) method was developed for the rapid identification and quantification of Leuconostoc mesnteroides and Lactobacillus plantarum populations which are the key microorganisms in kimchi fermentation. The strain-specific primers were designed to selectively amplify the target genes encoding 165 rRNA of L. plantarum and dextransucrase of L. mesenteroides. There was a linear relationship between the band intensity of PCR products and the number of colony forming units of each model organism. The PCR quantification method was compared with a traditional plate-counting method f3r the enumeration of the two lactic acid bacteria in a mixed suspension culture and also applied to a real food system, namely, watery kimchi. The population dynamics of the two model organisms in the mixed culture were reliably predictable by the competitive PCR analysis.

Keywords

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