Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Temperature, pH and Addition of Minerals in Lactic Acid Fermentation using Enterococcus faecalis RKY1.

Enterococcus faecalis RKYl을 이용한 젖산발효에서 온도, pH및 미량원소 첨가의 영향

  • 윤종선 (전남대학교 화학공학부, 생물산업기술 연구소) ;
  • 위영중 (전남대학교 화학공학부, 생물산업기술 연구소) ;
  • 오후록 (전남대학교 화학공학부, 생물산업기술 연구소) ;
  • 류화원 (전남대학교 화학공학부, 생물산업기술 연구소)
  • Published : 2002.09.01
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Abstract

In this study the effects of temperature, pH, and addition of some minerals were investigated in lactic acid fermentation using Enterococcus faecalis RKYI . As a result, strain of RKYl had retained biological activity at the wide range of temperature($34-46^{\circ}C$) and pH(6.0-9.0), and the optimum temperature and pH were $42^{\circ}C$ and 7.0, respectively. When the effect of various phosphate sources added on lactic acid fermentation was studied, di-basic forms of phos-phate(especially, ammonium phosphate and potassium phosphate) had more stimulating effect rather than mono-basic phosphate sources. And there were no perceivable effect of manganese and magnecium salts addition on lactic acid fermentation.

E. faecalis RKYl을 이용한 젖산발효에서 세포성장과 젖산 생산에 대한 배양온도 및 pH의 영향을 조사한 결과온도가 낮을수록 세포성장은 향상되었으나 발효시간이 지연되어 젖산생산이 저하되었으며$ 50^{\circ}C$에서는 세포성장이 거의 없었다. pH 7.0-9.0에서 거의 비슷한 세포성장 및 젖산생산을 보였으나 pH 6.0에서는 세포성장이 저하되었고 발효시간이 지연되었으며, pH 10.0에서는 세포성장이 거의 없었다. E. faecalis RKY에 의한 젖산발효는 비교적 넓은 범위의 배양온도($34- 46^{\circ}C$)및 pH(7.0-9.0)에서 안정하였으며 최적 배양온도 및 pH 는 각각 $42^{\circ}C$, 7.0이었다. 또한 일반적으로 일인산염에 비해 이인산염을 첨가해 주었을 때 젖산생산성이 향상되었으며, 효모추출물 10g·$L^{-1}$을 질소원으로 사용하여 배양하였을때 $Mg^{2+}$ 과 $Mn^{2+}$의 첨가효과는 없는 것으로 나타났다.

Keywords

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