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Identification and Characteristics of Penicillium spp. Isolated from Postharvest Decay of Pear

수확후 배 푸른곰팡이병을 일으키는 Penicillium속의 종류 및 특성

  • Kim, Ju-Hee (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Lee, Wang-Hyu (Faculty Biological Resources Science, Chononbuk National University) ;
  • Cheong, Seong-Soo (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Choi, Joung-Sik (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Ryu, Jeong (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Choi, Yeong-Geun (Jeollabuk-do Agricultural Research and Extension Services)
  • 김주희 (전라북도농업기술원) ;
  • 이왕휴 (전북대학교 농과대학 생물자원과학부 농업과학기술연구소) ;
  • 정성수 (전라북도농업기술원) ;
  • 최정식 (전라북도농업기술원) ;
  • 류정 (전라북도농업기술원) ;
  • 최영근 (전라북도농업기술원)
  • Published : 2002.08.01

Abstract

This study was carried out to identify the causal pathogens and investigated the characteristics of Penicillium spp. isolated from postharvest decay of pear. One hundred and ninety eight Penicillium spp. were isolated from infected pear fruits. The lesions were formed when the isolated pathogen were inoculated into the wounds and unwounds of pear fruit. Total isolates were classified into 15 groups by the size, color, pigment of colony and shape of conidia. These isolates were identified to be P.expansum, P.solitum, and P.crustosum according to the types of morphological, cultural and physiological characteristics. The pathogenicity was higher in wound inoculation at low temperature than unwound one. This result confirmed that wound promoted the disease appearance. P.expansum was appeared to have the most strong virulence, whereas P.solitum and P.crustosum were classified as weak virulent species by pathogenicity test on pear fruits.

수확 후 피해를 주는 Penicillium속의 병원체를 분리 동정하기 위하여 여러 특성을 조사하였다. 저장 중 배에서 분리한 198균주는 상처 접종과 무상 접종에서 병반을 형성하여 병원성이 있었으며, 균총 크기와 형태, 색, 배지뒷면 색소와 포자형태 등의 특성에 따라 15개 그룹으로 나눌 수 있었다. 분리한 균주들은 배양·형태적·생리적 특성에 따라 P. expansum, P. solitum, P. crustosum 등 3종으로 동정되었다. 분리된 균주들의 병원성 검정 결과 저온에서 상처접종이 무상접종 보다 병원성이 강하게 나타났으며 P. expansum이 저장 배 과실에 대해 가장 병원성이 강하였고, P. solitum과 P. crustosum은 약한 편이었다.

Keywords

References

  1. Agrios, G. N. 1997. Ptant pathology, 4rd. ed., 359-367. Academic Press. 635pp
  2. Bemy, J. F. and Hennebert, G. L. 1990. Variants of Penicillium expansum: An analysis of cultural and microscopic characters as taxonomic criteria; In: Modem concepts in PeniciUium and AspergiUus classification, eds. by Samson R. A. and Pitt, J. I., pp49-65. Plenum press, New York and London
  3. Frisvad, J. C. 1981. Physiological criteria and mycotoxin production as aids in identification of common asymmetric Penicillia. Appt. Environ. Microbiot. 41: 568-579
  4. 김용기, 김령희, 류재당, 류재기, 이상엽, 최용철. 1998. 사과저장병의 발생 및 방제.한국농약과학회지 2: 83-89
  5. 한국식물병리학회. 1998. 한국식물병명목록. 3th, 436pp
  6. Moss, M. O. 1987. Morphology and physiology of Penicillium and Acremonium In: BiotechnoIogy handbooks, eds, by Peberdy, J. R, pp. 37-65. Plenum press, New York
  7. Okuda, T. 1994. Variation in colony characteristics of Penicittium strains resulting from minor variation in culture conditions. Mycotoeia 86: 259-262
  8. Pitt, J. I. and Cruickshank, R. H. 1990. Speciadon and synonymy in Penicittium subgenus PeniciUiujm towards a definitive taxonomy In: Modem concepts in Penicittium and Aspergiltus classification, eds. by Samson, R. A. and J. I. Pitt, pp. 103-117. Plenum press, New York and London
  9. Ramirez, C. and Martinez. 1982. Manuat and atlas of the Penicittia. Elsevier Biomedical, New York. 974pp
  10. Stollc, A. C., Samson, R. A., Frisvad, J. C. and Filtenborg, O. 1990. The systematics of the terverticillte PeniciUia. In:Modem concepts in PeniciUium and AspergiItus classification, eds. by Samson, R. A. and Pitt, J. I. pp. 121-137. Plenum press, New York and London

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