Preventive Nutrition and Food Science

  • 제7권2호
  • /
  • Pages.128-132
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  • 2002
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  • 2287-1098(pISSN)
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  • 2287-8602(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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Apoptosis of MCF7 Cells Treated with PKC Inhibitors and Daunorubicin

  • Park, Won-Chul (Dept. of Biology, College of Natural Sciences, Pusan National University) ;
  • Son, Joo-Young (Dept. of Biology, College of Natural Sciences, Pusan National University) ;
  • Chung, Sook-Hyun (Dept. of Food Engineering, Dongseo University) ;
  • An, Woon-Gun (Dept. of Biology, College of Natural Sciences, Kyungpook National University)
  • 발행 : 2002.06.01
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초록

The present study was performed to observe the role of protein kinase C (PKC) inhibitors (H-7, staurosporine) and daunorubicin in the cell death process of MCF7 cells; and examined whether or not the type of induced cell death was apoptosis. The usefulness of the combined therapy of PKC inhibitors and daunorubicin to improve the adverse effect of daunorubicin was also investigated. Cell death was induced by treatment with PKC inhibitors or daunorubicin. Characteristic morphologic features of cell shrinkage, chromatic condensation, and cytoplasmic vacuolization were observed. These treatments also stimulated the cleavage of poly-(ADP-ribose) polymerase (PARP), an early event in apoptosis. With slight differences in the percentage of apoptosis-induced cells, staurosporine, H-7 and daunorubicin effectively induced apoptosis in MCF7 cells. Furthermore, combined treatment of PKC inhibitors and daunorubicin significantly drove the cells into an apoptotic state. Hence, our results revealed the possible therapeutic value of combined therapy for the prevention of drug resistance and adverse side effects.

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참고문헌

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