Biotechnology and Bioprocess Engineering:BBE

  • 제7권3호
  • /
  • Pages.178-184
  • /
  • 2002
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Characterization of the KG1a Cell Line for Use in a Cell Migration Based Screening Assay

  • Bernhard O. Palsson (Department of Bioengineering, University of California San Diego) ;
  • Karl francis (Department of Bioengineering, University of California San Diego) ;
  • Lee, Gyun-Min (KAIST, Department of Biological Sciences)
  • 발행 : 2002.05.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

High-throughput screening has become a popular method used to identify new “leads”for potentially therapeutic compounds. Further screening of these lead compounds is typically done with secondary assays which may utilize living, functioning cells as screening tools. A problem (or benefit) with these cell-based assays is that living cells are very sensitive to their environment. We have been interested in the process of stem cell migration and how it relates to the cellular therapy of bone marrow transplantation. In this study we describe a secondary, cell-based assay for screening the effects of various in-vitro conditions on Immature Hematopoietic Cell (IHC) migration. Our results have revealed many subtle factors, such as the cell's adhesive characteristics, or the effect of a culture's growth phase, that need to be accounted for in a screening protocol. Finally, we show that exponentially glowing KG1a cells (a human IHC cell line) were 10 times more motile than those in the lag or stationary phases. These data strongly suggest that KG1a cells secrete a chemokinetic factor during the exponential growth phase of a culture.

키워드

참고문헌

  1. Fluorescence Microscopy of Living Cells in Culture - Part A. Wang, Y.;D. L. Taylor
  2. Biotechnol. Bioeng. v.43 Analysis of Endothelial cell locomotion: Differential effects of motility and contact inhibition Lee, Y., L. V. McIntire;K. Zygourakis https://doi.org/10.1002/bit.260430712
  3. Internat. Rev. Cytol. v.163 The use of computers in understanding how animal cells crawl Soll, D. R. https://doi.org/10.1016/S0074-7696(08)62209-3
  4. Ph.D. Thesis. An Ex-Vivo Study of the Cellular Fate Process of Migration in Immature Hematopoietic Cells Francis, K.
  5. Blood v.56 An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1) Koeffler, H. P.;R. Billing;A. J. Lusis;R. Sparkes;D. W. Golde
  6. Blood v.62 Induction of differentiation of human acute myelogenous leukemia cells: Therapeutic implications Koeffler, H. P.
  7. J. Immunol. v.133 Antigenic analysis of hematopoiesis III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG1a Cells Civin, C. I.;L. C. Strauss;C. Brovall;M. J. Fackler;J. F. Schwartz;J. H. Shaper
  8. Blood v.87 CD34: Structure, biology, and clinical utility Krause, D. S.;M. J. Fackler;C. I. Civin;W. S. May
  9. Blood v.90 Adhesion and migration are differentially regulated in hematopoietic progenitor cells by cytokines and extracellular matrix Strobel, E.;D. Mobest;S. von Kliest;M. Dangel;S. Ries;R. Mertelsmann;R. Henschler
  10. J. Cell Biol. v.122 Maximal migration of human smooth muscle cells on fibronectin and type IV collagen occurs at an intermediate attachment strength DiMilla, P. A.;J. A. Stone;J. A. Quinn;S. M. Albelda;D. A. Lauffenburger https://doi.org/10.1083/jcb.122.3.729
  11. J. Cell Sci. v.98 Neural crest cell locomotion induced by antibodies to beta 1 integrins: A tool for studying the roles of substratum molecular avidity and density in migration Duband, J.;S. Dufour;S. Yamada;K. Yamada;J. Thiery
  12. Receptors: Models for Binding, Trafficking, and Signaling Lauffenburger, D. A.;J. J. Linderman
  13. J. Exp. Med. v.137 Leukocyte locomotion and chemotaxis Zigmond, S. H.;J. G. Hirsch https://doi.org/10.1084/jem.137.2.387
  14. J. Exp. Med. v.185 The chemokine SDF-1 is a chemoattractant for human CD34+ hematopoietic progeni-tor cells and provides a new mechanism to explain the mobilization of CD34+ progenitors to the peripheral blood Aiuti, A.;I. J. Webb;C. Bleul;T. Springer;J. C. Gutierrez-Ramos https://doi.org/10.1084/jem.185.1.111
  15. Proc. Soc. Exp. Bio. Med. v.147 A simple in-vitro method for studies on chemotaxis Cutler, J. E.
  16. J. Immunol. v.115 Chemotaxis under agarose: A new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes Nelson, R. D.;P. G. Quie;R. L. Simmons
  17. Biotechnol. Bioeng. v.36 Cell volume measurement as an estimation of mammalian cell biomass Frame, K.;W. Hu https://doi.org/10.1002/bit.260360211
  18. Biomed. Pharamacother. v.41 A critique of the methods used to assess leukocyte behaviour Lackie, J., N. Chaabane;K. Crocket