Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A New Spin Filter for High Density Culture and Ethanol Production by Saccharomyces cerevisiae

  • Moon, Hyun-Soo (Graduate School of Chemical Biotechnology, Korea University) ;
  • Lim, Dong-Joon (School of Chemical Engineering and Technology, Yeungnam University) ;
  • Song, Gu-Young (School of Chemical Engineering and Technology, Yeungnam University) ;
  • Kang, Hyun-Ah (Korea Research Institute of Bioscience and Biochnology) ;
  • Kim, Seung-Wook (Department of Chemical Engineering, Korea University) ;
  • Kim, Ik-Hwan (Graduate School of Chemical Biotechnology, Korea University) ;
  • Hong, Suk-In (Department of Chemical Engineering, Korea University)
  • Published : 2002.06.01
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Abstract

A new spin filter consisting of $50{\mu}m$ (nominal pore size) depth fitters rolled on a stainless steel grid was developed, using Saccharomyces cerevisiae as a model suspension cell to evaluate the spin filter performance. In a 1.8-1 fermentor with a rotation speed of 300 rpm and perfusion rate of 4 ml/min, a cell concentration of 49 g/l and ethanol concentration of 45 g/l from 100 g/l glucose could be obtained in a perfusion culture. The major mechanisms for cell separation used by the large-pore spin filter appeared to be centrifugal force and pivotal movement of the cells in the spin filter.

Keywords

References

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