Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Glycolipid Biosurfactants Produced by Pseudomonas aeruginosa D2D2 from Diesel-Contaminated Soil

  • MOON, HYE-JOON (Research Institute for Clean Technology, Myong-Ji University) ;
  • YOUNG-KUONG LIM (Department of Environmental Engineering and Biotechnology, Myong-Ji University) ;
  • HEE-SIK KIM (Environmental Microbiology R.U., Korea Research Institute for Bioscience and Biotechnology) ;
  • DAE-YOUNG KWON (Department of Environmental Engineering and Biotechnology, Myong-Ji University) ;
  • WOOK-JIN CHUNG (Department of Environmental Engineering and Biotechnology, Myong-Ji University)
  • Published : 2002.06.01
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Abstract

A biosurfactant-producing bacterial strain was selected from diesel-contaminated soil by measuring the oil-film collapsing activity and identified as Pseudomonas aeruginosa D2D2. When glucose and olive oil were used as carbon sources, 11.46 g/1 of biosurfactant was obtained. Based on TLC analysis, the biosurfactant produced from P. aeruginosa D2D2 was identified as a glycolipid, consisting of two types of biosurfactants (Type I and Type II). The purified glycolipid reduced the surface tension of the culture from 72 dyne/cm to 27 dyne/cm. The hydrophilic and hydrophobic moiety of the biosurfactant were rhamnose and ${\beta}$-hydroxydecanoic acid, as determined by FAB-MS and NMR analyses, respectively.

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References

  1. Biochem. J. v.330 Characterization of a cellobiose dehydrogenase from Humic insolens Charlotte, S.;H. Margrethe;S. Christensen;S. Martin https://doi.org/10.1042/bj3300565
  2. J. Microbiol, Biotechnol. v.9 Purification and characterization of biosurfactant from Tsukamurella sp. 26A Choi, K.S.;S. H. Kim;T. H. Lee
  3. Manual for the Identification of Medical Bacteria,2nd ed. Cowan, N. R.;K. J. Steel.
  4. PHYLIP(Phylogeny Inference Package)version 3.5c. Felsenstein, J.
  5. Agric. Biol. Chem. v.35 Formation of rhamnolipid by Pseudomonas aeruginosa and its function in hydrocarbon fermentation Hisatsuka, K.;T. Nakahara;N. Sano;K. Yamada https://doi.org/10.1271/bbb1961.35.686
  6. Korean J. Appl. Microbiol. Biotechnol. v.2 Surface-activity and environmental characteristics of biosurfactant produced by Pseudomenas aeruginosa JRT-4 Hwang, K.A.;J. R. Lee;S. J. Kim;Y.S. Kim;H.J. Ahn
  7. Inform. v.4 Biosurfactants face increasing interest Ishigami, Y.
  8. J. Bacteriol. v.179 Roles of Pseudomonas aeruginosa las and Quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes James, P.P.;C.P. Everett;B.H. Iglewski https://doi.org/10.1128/jb.179.18.5756-5767.1997
  9. Enzyme Microb. Technol. v.16 Microbially enhanced oil recovery (MEOR);Importance and mechanisms of MEOR Khire, J. M.;M. I. Khan https://doi.org/10.1016/0141-0229(94)90081-7
  10. Nucleic Acid Techniques in Bacterial Systematics Lane, D.J.
  11. J. Microbiol. Methods v.13 A drop-collapsing test for screening surfactant-producing microorganisms Jain, D. K.;D. L. Collins-Thomapson;H. Lee;J. T. Trevors https://doi.org/10.1016/0167-7012(91)90064-W
  12. J. Chrom. v.825 General approach for the development of high-performance liquid chromatography methods for biosurfactant analysis and purification Lin, S. C.;Y. C. Chen;Y. M. Lin https://doi.org/10.1016/S0021-9673(98)00709-2
  13. J. Microbiol. v.5 Production of extracellular emulsifying agent by Pesudomenas aeruginosa UGI MacElwee, C. G.;H. Lee;J. T. Trevors
  14. Biotech. Lett. v.11 Effect of the carbon source on biosurfactant production by Pseudomonas aeruginosa 44T1 Robert, M.;M. E. Mercade;M. P. Bosch;J. L. Parra;M. J. Espuny;M. A. Manresa;J. Guinea https://doi.org/10.1007/BF01026843
  15. Biochemical Tests for Identification for Medical Bacteria, 2nd ed. MacFaddin, J. F.
  16. FEMS Microbiol. Lett. v.100 DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities Rochelle, P.A.;J. C. Fry;R. J. Parkes;A. J. Weightman https://doi.org/10.1111/j.1574-6968.1992.tb05682.x
  17. Surfactants and Interfaciol Phenomena Rosen, M. J.
  18. J. Ind. Microbiol. Biotechnol. v.19 Production and characterization of a biosurfactant isolated from Pseudomonas aeruginosa UW-1 Sim, L.;O. P. Ward;Z. Y. Li https://doi.org/10.1038/sj.jim.2900450
  19. J. Microbiol. Biotechnol. v.9 Purification and characterization of biosurfactants produced by Pseudomonas sp.SW1. Suk, W. S.;H. J. Son;G. Lee;S. J. Lee
  20. Appl. Env. Microbiol. v.58 Enghanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant Zhang, Y.;R. M. Miller