YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

In vitro Modulation of Proliferation and Melanization of B16/F10 Melanoma Cells by Quercetin

Quercetin이 B16/F10 멜라닌세포의 중식 및 멜라닌화에 미치는 영향

  • 천현자 (원광대학교 한의학전문대학원) ;
  • 백승화 (원광대학교 한의학전문대학원) ;
  • 우원홍 (원광대학교 한의학전문대학원) ;
  • 황상구 (원광대학교 한의과대학 병리학교실) ;
  • 김춘관 (원광대학교 한의과대학 병리학교실) ;
  • 김춘관 (원광대학교 한의과대학 병리학교실)
  • Published : 2002.02.01
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Abstract

Quercetin is one of the bioflavonoid compounds and has multiple biological effects such as antioxidant and effective anti-inflammatory agent. Melanin has an important role in protecting human skin from the damaging effects of ultra-violet W) radiation. We studied the effect of quercetin on proliferation of B16/F10 melanoma cells. After 48h treatment of cells with quercetin, the cells exhibited a dose-dependent inhibition in their proliferation without apoptosis. Therefore, the decrease in cell numbers may be due to cell growth arrest, not due to cell death by cytotoxicity. We also investigated the effect of quercetin on melanogenesis of this cells. B16/F10 melanoma cells were grown for 48h in the presence of 0.01~50$\mu\textrm{g}$/ml quercetin and the total melanin contents were measured. Quercetin stimulated melanization of the cells in low concentrations (0.01~20$\mu\textrm{g}$/ml), whereas it inhibited melanization in high concentrations (30~50$\mu\textrm{g}$/ml). It was observed that quercetin differently regulates melanogenesis of B16/F10 melanoma cells dependent on its concentrations.

Keywords

References

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