열역학법을 이용한 DNA hybridization 특성 검출 및 해석

Detection and Analysis of DNA Hybridization Characteristics by using Thermodynamic Method

  • 발행 : 2002.06.01

초록

The determination of DNA hybridization reaction can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and application area. So, the improvement of DNA hybridization detection method is very important for the determination of this hybridization reaction. Several molecular biological techniques require accurate predictions of matched versus mismatched hybridization thermodynamics, such as PCR, sequencing by hybridization, gene diagnostics and antisense oligonucleotide probes. In addition, recent developments of oligonucleotide chip arrays as means for biochemical assays and DNA sequencing requires accurate knowledge of hybridization thermodynamics and population ratios at matched and mismatched target sites. In this study, we report the characteristics of the probe and matched, mismatched target oligonucleotide hybridization reaction using thermodynamic method. Thermodynamic of 5 oligonucleotides with central and terminal mismatch sequences were obtained by measured UV-absorbance as a function of temperature. The data show that the nearest-neighbor base-pair model is adequate for predicting thermodynamics of oligonucleotides with average deviations for $\Delta$H$^{0}$ , $\Delta$S$^{0}$ , $\Delta$G$_{37}$ $^{0}$ and T$_{m}$, respectively.>$^{0}$ and T$_{m}$, respectively.

키워드

참고문헌

  1. J. D. Watson, F. H. C. Crick, 'Molecular structure of nucleic acids: a structure for deoxyribose nucleic acid', Nature, vol. 171, pp.737-738, 1953
  2. J. D. Watson, F. H. C. Crick, 'Genetic implications of the structure of deoxyribose nucleic acid', Nature, vol. 171, p.964, 1953
  3. H. R. Wilson, 'The double helix and all that', Trends Biochem. Sci., vol. 13, pp.275-278, 1988 https://doi.org/10.1016/0968-0004(88)90163-6
  4. Benjamin Lewin, 'GENES V', Oxpord University Press and Cell Press, 1994, pp.89-90
  5. Hatim T., Allawi and John SantaLucia, Jr., 'Thermodynamics and NMR of Internal G.T Mismatches in DNA', Biochemistry, vol. 36, pp.10581-10594, 1997 https://doi.org/10.1021/bi962590c
  6. N. Sugimoto, S. Nakano, M. Yoneyama, 'Thermodynamics-structure relationship of single mismatches in RNA/DNA duplexes', Biochemistry, vol. 39, pp.11270-11281, 2000 https://doi.org/10.1021/bi000819pS0006-2960(00)00819-9