Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Cloning and characterization of the multiprotein bridging factor 1 (YIMBFI) gene from the dimorphi yeast Yarrowia lipolytica

  • Kim, Jeong-Yoon (Department of Microbiology, Chungnam University, DaeJeon 305-764) ;
  • Kim, Jang-Hwan (Department of Microbiology, Chungnam University, DaeJeon 305-764) ;
  • Cheon, Seong-Ah (Department of Microbiology, Chungnam University, DaeJeon 305-764) ;
  • Yunkyoung Song (Department of Microbiology, Chungnam University, DaeJeon 305-764)
  • Published : 2002.06.01
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Abstract

In order to identify Yawowia lipolytica genes induced by serum, cDNA representational difference analysis was performed using a PCR-select CDNA subtraction method. One of the genes cloned from the subtraction was a gene (YIMBFl) homologous to Saccharomyces cerevisiae MBF1 encoding the coactivator multiprotein bridging factor 1. Disruption of YIMBFl revealed that the gene was net essential for viability, and the Ylmbf△ strain did not show any distinct phenotypic change on solid serum medium. In liquid medium, however. a difference was found in the ability to maintain hyphae induced by serum. This result suggests that the YIMbf1 protein may mediate transcriptional activation of certain genes involved in the hypha fonmation of Y. lipolytica.

Keywords

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