Isolation, Identification and Characterization of Ynncomycin-Resistant Enterococci from Raw Milk

  • Ha, Nam-Joo (Department of Pharmacy, Sahmyook University, Seoul 139-742) ;
  • Cho, Sung-Sook (Department of Food Science, Sahmyook College, Seoul 139-742) ;
  • Kim, Bong-Su (Laboratory of Antimicrobial Resistant Pathogens, National Institute of Health, Seoul 122-71)
  • Published : 2002.06.01

Abstract

To determine the occurrence of vancomycin-resistant Enterococci in a raw milk sample, raw milk samples were examined for a period of 6 months. Enterococci were isolated directly from Enterococcal selective agar plates supplemented with 2 mg of vancomycin per liter, Nineteen strains were selected and identified by applying the Vitek system. To determine resistance patterns, 19 isolates were tested with vancomycin and teicoplanin. Vancomycin-resistant Enterococci were genotyped by using a PCR analysis and 5 out of 19 isolates were of the VanC type.

Keywords

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