Journal of Microbiology

  • 제40권2호
  • /
  • Pages.156-160
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  • 2002
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Expression of the Galactokinase Gene (gaIK) from Lactococcus lactis asp. lactis ATCC7962 in Escherichia coil

  • Lee, Hyong-Joo (Department of Food Science and Technology, Seoul National University, Suwon 441-774) ;
  • Lee, Jung-Min (Department of Food Science and Technology, Seoul National University, Suwon 441-774) ;
  • Park, Jae-Yeon (Department of Food Science and Biotechnology, Kyonggi University, Suwon 442-760) ;
  • Lee, Jong-Hoon (Department of Food Science and Biotechnology, Kyonggi University, Suwon 442-760) ;
  • Kim, Jeong-Hwon (Department of food Science and Technology, Gyeongsang National University, Chinju 660-701) ;
  • Chang, Hea-Choon (Department of Food Nutrition, Chosun University, Kwamgiu 501-701) ;
  • Chung, Dae-Kyun (Institute and Department of Genetic Engineering, Kyunghee University, Suwon 449-701) ;
  • Kim, Somi-Cho (Subtropical Horticulture Research Center, Cheju National University, Cheju 690-756)
  • 발행 : 2002.06.01
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초록

The whole gal/lae operon genes of Lactococcus lactis ssp. lactis 7962 were reported as follows: galA-galM-galK-galT-lacA -lacZ-galE. The galK gene encoding a galactokinase involved in one of the Leloir pathways for galactose metabolism was found to be 1,197 bp in length and encodes a protein of 43,822 Da calculated molecular mass. The deduced amino acid sequence showed over 50% homology with GaIK proteins from several other lactic acid bacteria. The galK gene was expressed in E. coli and the product was identified as a 43 kDa protein which corresponds to the estimated size from the DNA sequence. The galactokinase activity of recombinant 5. coli was about 8 times greater against that of the host strain and more than 3 times higher than the induced L. lactis 7962.

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참고문헌

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