KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation Of Latobacillus Producing Exopolysaccharide and Optimization of its Production

Exopolysaccharide생산 유산균주의 분리 및 배양조건

  • 배인휴 (순천대학교 농과대학 동물자원학과) ;
  • 허정원 (경기도 보건환경연구원)
  • Published : 2002.04.01
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Abstract

A lactic acid bacterial isolate Lactobacillus ssp. SCU-M which produces exopolysaccharide was identified and its cultural Condition was investigated. The optimum Conditions for exopolysaccharide(EPS) Production Of Lactobacillus ssp. SCU-M were 37$\^{C}$, pH 6.5, using medium composed of 1.5% galactose, 1.0% yeast extract, 0.25% peptone, 0.15% MgSO$_4$, 0.15% K$_2$HPO$_4$ and 0.1% tween 80 in distilled water. The EPS concentration after 48 hours at the Initial pH 6.5, 37$\^{C}$ in a flask culture was 1,680 mg/ℓ.

원유로부터 다당류를 생산하는 유산균을 분리, 선발하여 균의 배양조건을 조사하고 Lactobacillus ssp. SCU-M으로 동정, 명명된 최적배지의 탄소원은 galactose 1.5% 첨가시 553 mg/L의 EPS를 생성하였으며 whey의 이용성 검토를 위한 deproteinized whey와 lactic acidified whey를 탄소원으로 사용하였을 때 각각 639, 550 mg/L를 생성하였다. 질소원으로 yeast extract 1.5%, peptone 0.5%를 첨가한 경우 934 mg/L, 미량성분으로 , 0.15% $K_2$HPO, 0.15% 첨가시 1,076 mg/L의 다당 생산성을 나타내었다. 따라서 이들 결과를 종합하여 galactose 1.50%, yeast extract 1.00%, peptone 0.25%, MgSO$_4$, 7$H_2O$ 0.15%, $K_2$HPO$_4$0.15%, tween 80, 0.10%를 적정 배지로 하여 PH 6.5, 배양온도 37$^{\circ}C$에서 배양시 1,680 mg/L의 EPS를 생성하였다.

Keywords

References

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