Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Expression of Taurine Transporter in Cell Lines and Murine Organs

세포주와 마우스 조직에서 타우린수송체의 발현분석

  • 김하원 (서울시립대학교 생명과학과) ;
  • 안희창 (서울시립대학교 생명과학과) ;
  • 안혜숙 (서울시립대학교 생명과학과) ;
  • 현진원 (제주대학교 의과대학) ;
  • 이은방 (서울대학교 천연물과학연구소)
  • Published : 2002.06.01
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Abstract

Taurine (2-ethaneaminosulfonic acid, $^+{NH}_3{CH_2}{CH_2}{SO_3^{-}}$) is endogenous amino acid with functions as modulator of osmoregulation, antioxidation, detoxification, transmembrane calcium transport, and a free radical scavenger in mammalian tissues. Taurine transporter(TAUT) contains 12 transmembrane helices, which are typical of the $Na^+$- and $Cl^-$-dependent transporter gene family, and has been cloned recently from several species and tissues. To analyze the expression of TAUT mRNA, one step RT-PCR was performed from human and mouse cultured cell lines and from various mouse tissues. The primers were designed to encode highly conserved amino acid sequences at the second transmembrane domain and at the fourth and fifth intracellular domains. RT-PCR analysis showed both of the human intestine HT-29 and mouse macrophage RAW264.7 cell lines expressed mRNA of TAUT. To define the expression patterns of the TAUT mRNA in the murine organs, RT-PCR was performed to detect cDNA representing TAUT mRNA from seven different mouse tissues. The TAUT was detected in all of the mouse tissues analyzed such as heart, lung, thymus, kidney, liver, spleen and brain. A large amount of transcript was fecund from heart, liver, spleen, kidney, and brain, while lung contained a very small amount of transcript.

Keywords

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