Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
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Expression of Intercellular Adhesion Molecule- 1 after Ischemia Reperfusion Injury of the Canine Lung

폐장의 허혈-재관류 손상과 세포간부착물질-1 의 발현

  • 성숙환 (서울대학교병원 흉부외과, 서울대학교 의과대학 흉부외과학교실) ;
  • 김영태 (서울대학교병원 흉부외과, 서울대학교 의과대학 흉부외과학교실) ;
  • 김문수 (서울대학교병원 흉부외과, 서울대학교 의과대학 흉부외과학교실) ;
  • 이재익 (서울대학교병원 흉부외과, 서울대학교 의과대학 흉부외과학교실) ;
  • 강문철 (서울대학교병원 흉부외과, 서울대학교 의과대학 흉부외과학교실)
  • Published : 2002.02.01
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Abstract

Background: Predicting the important role of intercellular adhesion molecule-1 expression on the acute ischemia-reperfusion injury, we set out to demonstrate it by assessing the degree of expression of ICAM-1 after warm ischemia-reperfusion in canine unilateral lung ischemia model. Material and Method: Left unilateral lung ischemia was induced by clamping the left hilum for 100 minutes in seven adult mongrel dogs. After reperfusion, various hemodynamic pararmeters and blood gases were analyzed for 4 hours, while intermittently clamping the right hilum in order to allow observation of the injured Ieft lung function. The pulmonary venous blood was collected serially to measure TNF- and cICAM-1 level. After 4 hours of reperfusion, the lung tissue was biopsied to assess cICAM-1 expression, and to measure tissue malondialdehyde(MDA) and ATP level. Result: The parameters including arterial oxygen partial pressure, pulmonary vascular resistance and tissue MDA and ATP level suggested severe lung damage. Serum TNF-$\alpha$ level was 8.76$\pm$2.37 ng/ml at 60 minutes after reperfusion and decreased thereafter. The cICAM-1 level showed no change after the reperfusion during the experiment. The tissue cICAM-1 expression was confirmed in 5 dogs. Conclusion: The increase of TNF-$\alpha$ Ievel and expression of tissue ICAM-1 were demonstrated after ischemia reperfusion injury in canine lung model.

배경: 폐장 이식 수술 시 필연적으로 1~4 시간 이내에 나타나는 급성 허혈-재관류 손상 기 전에 세포간부착물질의 활성화가 중요한 역할을 하리라는 가설을 세우고 동물실험을 통해 폐장에서 허혈-재관류를 유도한 후 세포간부착물질의 활성화 정도와 장기의 기능을 동시에 평가하여 실제로 세포간부착물질의 증가에 의한 염증반응과 장기 기능 저하 현상 사이에 밀접한 관계가 있는지를 증명하고자 하였다. 대상 및 방법: 체중 15~20kg의 잡견 7마리를 사용하여 좌측 폐문부를 차단하여 좌측 폐 온혈 허혈을 100분간 유발시킨 후 재관류를 시켰다. 재관류 후 4시간 동안 우측 폐문부를 간헐적으로 차단하면서 허혈시킨 좌측 폐 단독으로의 가스교환능, 혈역학적 변수, 호흡 역학적 변수를 측정하고, 각 시간대별로 폐정맥혈 내 TNF-$\alpha$및 cICAM-1 농도를 측정하였다. 실험 종료 후 폐 조직 수분 함량, 조직 내 지방 산화물과 (malonedialdehide; MDA) ATP양을 측정하고, 광학 현미경 소견 및 면역 형광염색법으로 혈관내피세포의 ICAM-1 발현 양상을 관찰하였다. 결과: 동맥혈 산소 분압, 폐혈관저항 및 조직검사, 조직 내 MDA 농도와 ATP 농도는 폐손상 소견을 나타내었다. TNF-$\alpha$는 재관류 1시간에 8.76$\pm$2.37 ng/ml으로 최고치를 보인 후 빠르게 감소하였으나 cICAM-1은 변화가 없었다, 면역현광염색법을 이용한 혈관내피세로의 ICAM-1은 변화가 없었다. 면역형광염색법을 이용한 혈관내피세포의 ICAM-1염색은 2마리에서는 허혈 전, 후의 차이가 발견되지 않았으나, 3마리에서는 허혈-재관류의 손상 후 ICAM-1의 발현이 관찰되었고, 2마리에서는 그 발현 양상이 허혈 전에 비해 매우 뚜렷하였다. 결론: 이상의 결과에서 폐장의 허혈-재관류 손상 시 싸이토카인 과 세포부착물질이 증가하나 그 증가 시기에 차이가 있음을 밝혔다.

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References

  1. Surgery v.111 Reduction by superoxide dismutase of leukocyte-endothelial adherence after liver transplatation Marzi I;Knee J;Buhren V;Menger M;Trentz O
  2. Hepatology v.18 Differential impact of Carolina rinse and University of Wisconsin solutions on microcirculation, leukocyte adhesion, Kupffer cell activity, and biliart excretion after liver transplantation Post S;Palma P;Rentsch M;Gonzalrz AP;Menger MD
  3. Transplantation v.55 Effects of Carolina rinse and adenosine on microvascular perfusion and intrahepatic leukocyte-endothelial interaction after liver transplantation in the rat Post S;Rentsch M;Gonzalez AP;Palma P;Otto G;Menger MD
  4. Transplant Proc v.27 Involvement of ICAM-1 expression on sinusoidal endothelial cell and neutrophil adherence in the reperfusion injury of cold-preserved livers Monden K;Arii S;Ishiguro S;et al
  5. Transplant Immunol v.1 The molecular immunology of acute rejection:an overview Halloran PF;Broski AP;Batiuk TD;Masrenas J
  6. Ann Surg v.219 Adhesion moleucules and transplantation Heemann UW;Tullius SG;Azuma H;Kupiec-Weglinsky J;Tilney NL
  7. Cell v.51 Purified intercellular adhesion molecule-1(ICAM-1) is a ligand for lymphocyte function-associated antigen-1(LFA-1) Marlin SD;Springer TA
  8. Cell v.65 Binding of thr integrin Mac-1(CD11b/CD18) to the third immunoglobulin-like domain of ICAM-1(CD54) and its regulation by glycosylation Diamond MS;Staunton DE;Mallin SD;Springer TA
  9. Am J Physiol v.263 Microvascular ischemia-reperfusion injury in striated muscle: significance of 'no-reflow' Menger MD;Steiner D;Messmer K
  10. Am J Pathol v.145 Hepatic Microcirculatory perfusion failure is a determinant of liver dysfunction in warm ischemia-reperfusion Vollmar B;Glasz J;Leiderer R;Post S;Menger MD
  11. Am J Physiol v.263 Micro-vascular ischemia-reperfusion injury in striated muscle: significsnce of 'reflow-paradox' Menger MD;Pelikan S;Steiner D;Messmer K
  12. Am J Physiol v.255 Role of xanthine oxidase and granulocytes in ischemia-reperfusion injury Granger DN
  13. Am J Physiol v.257 Leukocyte adherence to venular endothelium during ischemia-reperfusion Granger DN;Benoit JN;Suzuki M;Grisham MB
  14. Am J Physiol v.267 Impact of leukocyte-endothelial cell interaction in hepatic ischemia-reperfusion injury Vollmar B;Menger MD;Glasz J;Leiderer R;Messmer K
  15. FASEBJ v.4 Neutrophils contribute to ischemia-reperfusion injury in rat liver in vivo Jaeschke H;Farhood A;Smith CW
  16. Circ Res v.63 Granulocytes and no-refloe Phenomenon in irreversible hemorrhagic shock Barroso-Aranda J;Schmid-Schonbein GW;Zweifach BW;Engler RL
  17. Am J Physiol v.267 Role of MAc-1 and ICAM-1 in ischemia-reperfusion injury in a microcirculation model of BALB/C-mice Nolte D;Hecht R;Schmid P;et al
  18. Surgery v.117 Leukocytes contribute to hepatic ischemia/reperfusion injury via intercellular adhesion molecule-1-mediated venular adherence Vollmar B;Glasz J;Menger MD;Messmer K
  19. J Cell Biol v.112 Oxygen radicals induce human endothelial cells to express GMP-140 and bind neutrophils Patel KD;Zimmerman GA;Prescott SM;McEver RP;McIntyre TM
  20. J ExpMed v.180 Neutrophils roll on adherent neutrophils bound to cytokine-induced endothelial cells via L-selsctin on the rolling cells Bargatae RF;Kurk S;Butcher EC;Jutila MA
  21. J Heart Lung Transpant v.13 Survival in lung reperfusion injury is im proved by an antibody that binds and inhibits L-and E-selectin Steinberg JB;Mao H;Niles SD;Jutila MA;Kapelanski DP
  22. Transplantation v.62 Attenuation of anti-ICAM-1 and anti-β2 integrin monoclonal antibodies DeMeester SR;Molinari MA;Shiraishi T;et al
  23. Shock v.10 Tumor necrosis fatcor up-regulates intercellular adhesion molecule 1,which is important in the neutrophil-dependent lung and liver injury associated with hepatic ischemia and reperfusion injury Colletti LM;Cortis A;Lukacs N;et al
  24. Transplantation v.58 Tumor necrosis factor-alpha and interferon-gamma sfter ischemia reperfusion injury in the lung allograft Colletti LM;Cortis A;Lukacs N;et al
  25. Am J Respir Crit Care Med v.152 tumor necrosis factor-alpha, and interferon-gamma release after ischemia reperfusion injury in a novel lung autograft animal model Serrick C;Franchesca SL;Giaid A;Shennib H;Cytokine interleukin-2
  26. J Appl Physiol v.85 Tumor necrosis factor-alpha in ischemia and reperfusion injury in rat lungs Khimenko PL;Bagby GJ;Fuseler J;Taylor AE
  27. J Heart Lung Transplant v.14 Expression of intercellular and vascular cell adhesion molecules and class Ⅱ major histocompatibility antigens in human lungs Hasegawa S;Ritter JH;Patterson GA;et al
  28. Am J Pathol v.143 Expression of intercellular adhesion molecule-1 in rat heart with ischemia reperfusion and limitation of infarct size by treatment with antibodies against cell adhesion molecules Yamazaki T;Seko Y;Tamatani T;et al
  29. Am J Physiol v.267 Role of Mac-1 and ICAM-1 in ischemia-reperfusion injury in a microcir-culation model of BALB/C mice Nolte D;Hecht R;Schmid P;et al