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Effects of Resistant Starch on Gut Functions and Plasma Lipid Profiles in Rats Fed High Fat Diet

난소화성전분의 섭취가 고지방식이를 섭취한 흰쥐에서 장 기능과 혈액지방에 미치는 영향

  • 정미경 (단국대학교 식품영양학과) ;
  • 김명환 (단국대학교 식품공학과) ;
  • 강남이 (서울보건대학 식품영양과) ;
  • 김우경 (단국대학교 식품영양학과)
  • Published : 2002.04.01

Abstract

We have investigated the intestinal functions and hypolipidemic effects of resistant starch (RS) in rats. Experimental groups were CON (cooked starch 45% + RS 7%), RSIO (cooked starch 35% +RS 10%), RS20 (cooked starch 25%+RS20%), and RS30 (cooked starch 15%+RS 30%). The weight gains during experimental period were slightly decreased by intake of resistant starch and the weights of epididymal fat pad were lower in resistant starch intake groups than in CON, although the difference was not significant. In intestinal functions, water contents of fecal, transit time and colon cell proliferation were affected by resistant starch. Plasma total lipid and triglyceride concentrations were significantly decreased, dose-dependently, by resistant starch intake. Conclusively, it is important to intake resistant starch in order to decrease plasma lipids and to improve intestinal functions.

본 연구는 고지방식이 섭취시 RS섭취가 장기능 및 혈액지방성분에 미치는 영향을 알아보고자 하였다. 생후 4주된 실험동물을 구입하여 6주간 고지방식이로 사육하여 성장 시킨 후 RS함량이 다른 실험식이로 4주간 더 사육한 후 실험한 결과는 다음과 같다. 4주간의 실험식이를 섭취하였을 때 식이 섭취량과 식이효율은 실험군간의 차 가 없었고, 대조군에 비해 RS30군에서 체중과 부고환지방무게가 유의적이지는 않으나 감소하는 경향이었다. 소장과 대장의 길이, 맹장의 무게는 실험군간의 유의적인 차이가 없었다. 분변내 수분함량을 보면 RS를 30%섭취한 군에서 유의적이지는 않으나 증가하였고, 실험식이 섭취후 3주째 측정한 장통과시간은 RS 30군에서 감소하는 경향을 보였다. 대장세포의 증식은 RS30 군에서 유의적이지는 않으나 증가하는 경향을 보였다. RS섭취량에 따라 총지방량, 중성지방량은 유의적으로 감소하였으나 콜레스테롤과 HDL-콜레스테롤량은 실험군간에 차이가 없었다. 이상의 결과에서 고지방을 섭취한 성장이후의 쥐에서 RS섭취는 유의적 이지는 않으나 체중과 체내 저장지방, 장통과시간을 감소시키는 경향을 보였으며 , 혈액 내 총지방량과 중성지방량을 유의적으로 감소시켰다. 그러므로 난소화성전분의 섭취는 저열량원으로의 가능성을 보여주고 있으며 , 고지방식이시 혈액내 총지방량과 중성지방을 낮추는 효과가 있는 것으로 사료된다 그러나 이러한 효과는 dose dependent 하지는 않았으며 난소화성 전분을 식이무게의 30%로 섭취할 경우에 유의적으로 나타났다.

Keywords

References

  1. Englyst HN, Trowell H, Southgate DAT, Cummings JH. 1987. Dietary fiber and resistant starch. Am J Clin Nutr 46: 873-874. https://doi.org/10.1093/ajcn/46.6.873
  2. Euresta (European Flair Concerted Action on Resistant starch). 1993. Newsletter IV. Department of Human Nutrition. Wageningen Agriculture University.
  3. Bjorck I, Nyman M, Pedersen B, Siljestrom M, Asp NG, Eggum BO. 1987. Formation of enzyme resistant starch during autoclaving of wheat starch: studies in vitro and in vivo. J Cereal Sci 6: 159-165. https://doi.org/10.1016/S0733-5210(87)80052-8
  4. Tovar J, Bjorck I, Asp NG. 1992. Starch content and alpha-amylolysis rate in precooked legume flours. J Agric Chem 38: 1818-1823. https://doi.org/10.1021/jf00099a007
  5. Sievert D, Pomeranz, Y. 1989. Enzyme-resistant starch I. Characterization and evaluation by enzymatic, thermoanalytical and microscopic methods. Cereal Chem 66: 342-347.
  6. Haralampu SG. 2000. Resistant starch-a review of physical properties and biological impact of $RS_3$. Carbohydrate Polymers 41: 285-292. https://doi.org/10.1016/S0144-8617(99)00147-2
  7. Behall Km, Howe JC. 1996. Resistant starch as energy. J Food Sci Tech 36: 355-357.
  8. Brown I, Warhust M, Arcot J, Playne M, Illman RJ, Topping DL. 1997. Fecal numbers of bifidobacteria are higher in pigs fed Bifidobacterium longum with a high amylose cornstarch than with a low amylose cornstarch. J Nutr 127: 1822-1827. https://doi.org/10.1093/jn/127.9.1822
  9. de Deckere EAM, Kloots WJ, van Amelsvoort JM. 1993. Resistant starch decreases serum total cholesterol and triglycerol concentrations in rats. J Nutr 123: 2142-2151.
  10. Topping DL, Clifton PM. 2001. Short-chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiol Rev 81: 1031-1064. https://doi.org/10.1152/physrev.2001.81.3.1031
  11. Reeves PG, Nielsen FH, Fahey GC. 1993. AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet. J Nutr 123: 1939-1951. https://doi.org/10.1093/jn/123.11.1939
  12. Park SH, Lee TK, Lee HS. 1994. The effect of dietary fiber feeding on gastrointestine functions and lipid and glucose metabolism in streptozotocin-induced diabetic rats. Kor J Nutr 27: 311-322.
  13. Schutte B, Reynders MMJ, Bosman FT, Blijham GH. 1987. Studies with antibromodeoxyuridine antibodies II: Simultaneous immunocytochemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa. J Histochem Cytochem 35: 371-374. https://doi.org/10.1177/35.3.3546484
  14. Frings CS, Dunn RT. 1970. A colorimetric method for determination of total serum lipids based on the sulfphosphovanillin reaction. Am J Chin Pathol 53: 89-91. https://doi.org/10.1093/ajcp/53.1.89
  15. Zak B. 1968. Total and free cholesterol. In Standard method chemistry. Academic Press, New York. p 79-89.
  16. Folch JM, Lees G, Stanley HS. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Bio Chem 223: 497-509.
  17. de Deckere EAM, Kloots WJ, van Amelsvoort JM. 1995. Both raw and retrograted starch decrease serum triglycerol concentration and fat accretion in the rat. Br J Nutr 73: 287-298. https://doi.org/10.1079/BJN19950030
  18. Cummings JH, Beatty ER, Kingman SM, Bingham SA, Englyst HN. 1996. Digestion and physiological properties of resistant starch in the human large bowel. Br J Nutr 75: 733-747. https://doi.org/10.1079/BJN19960177
  19. Schrijver R, Vanhoof KM, Vande GJ. 1999. Nutrient utilization in rats and pigs fed enzyme resistant starch. Nutr Res 19: 1349-1361. https://doi.org/10.1016/S0271-5317(99)00092-5
  20. Murphy O. 2001. Non-polyol low-digestible carbohydrates: food application and functional benefits. Brit J Nutr 85: S47-S53. https://doi.org/10.1079/BJN2000261
  21. Lopez HW, Coudray C, Bellanher J, Levrat-verny MA, Demigne C, Rayssiguier Y, Remesy C. 2000. Resistant starch improves mineral assimilation in rats adapted to a wheat bran diet. Nutr Res 20: 141-155. https://doi.org/10.1016/S0271-5317(99)00146-3
  22. Olsen M, Rumessen JJ, Gudmand-Hoyer E. 1991. Large bowel fermentation in rats eating processed potatoes. J Nutr 66: 313-329. https://doi.org/10.1079/BJN19910034
  23. Phillips J, Muir J, Birkett A, Lu ZX, Jones GP, O'Dea K, Young GP. 1995. Effect of resistant starch on fecal bulk and fermentation dependent events in humans. Am J Clin Nutr 62: 121-130. https://doi.org/10.1093/ajcn/62.1.121
  24. Brunsgaard G, Eggum BO, Standstrom B. 1995. Gastrointestinal growth in rats as influenced by indigestible polysaccharides and adaptation period. Comp Biochem Physiol 111A: 369-377.
  25. Ferguson LR, Tasman-Jones C, Englyst H, Harris PJ. 2000. Comparative effects of three resistant starch preparations on transit time and short chain fatty acid production in rats. Nutr Cancer 36: 230-237 https://doi.org/10.1207/S15327914NC3602_13
  26. Bradley WA, Gianturco, SH. 1994. Triglyceride-rich lipoproteins and atherosclerosis: pathophysiological considerations. J Int Med 361: 33-39.
  27. Reiser S, Powell AS, Scholfield DJ, Panda P, Ellwood KC, Canary JJ. 1989. Blood lipids, lipoproteins, apoproteins and uric aicd in men fed diets containing high fructose or high amylose cornstarch. Am J Clin Nutr 49: 832-839. https://doi.org/10.1093/ajcn/49.5.832
  28. Fernandez ML, Roy S, Vergara-Jimenez M. 2000. Resistant starch and cholestyramine have distinct effects on hephatic cholesterol metabolism in guinea pigs fed a hypercholesterolmic diet. Nutr Res 20: 837-849. https://doi.org/10.1016/S0271-5317(00)00170-6
  29. Morand C, Levart MA, Besson C, Demigne C, Remesy C. 1994. Effects of diet rich in resistant starch on hepatic lipid metabolism in rats. J Nutr Biochem 5: 138-144. https://doi.org/10.1016/0955-2863(94)90085-X
  30. Ranhotra GS, Gelroth JA, Leinen SD. 1997. Hypolipidemic effects of resistant starch in hamsters is not dose dependent. Nutr Res 17: 317-323. https://doi.org/10.1016/S0271-5317(96)00261-8
  31. Fernandez ML, Ruiz LR, Conde AK, Sun DM, Erickson SK, McNamara DJ. 1995. Psyllium reduces plasma LDL in guinea pigs by altering hepatic cholesterol homeostasis. J Lipid Res 36: 1128-1138.
  32. Younes H, Levrat M, Demigne C, Remest C. 1995. Resistant starch is more effective than cholestyramine as a lipid-lowering agent in the rats. Lipids 30: 847-853. https://doi.org/10.1007/BF02533961

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