Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지

Protective Effect of Water Extract of Lycii Cordex Radicis on Lipid Peroxidation of Rat Skin Exposed to Ultraviolet B Radiation

자외선 B에 노출된 쥐 표피의 지질과산화에 대한 지골피 물 추출물의 보호효과

  • Gwak, Jun-Su (Department of Korean Traditional Medicines Science, Masan College) ;
  • Ryu, Seung-Hee (School of Food Science, Inje University) ;
  • Moon, Gap-Soon (School of Food Science, Inje University) ;
  • Choi, Dong-Seong (Division of Bioscience and Biotechnology, Woosuk University) ;
  • Park, Sung-Hye (Professional Graduated School of Oriental Medicine, Wonkwang University) ;
  • Han, Jong-Hyun (Professional Graduated School of Oriental Medicine, Wonkwang University) ;
  • Ahn, Byung-Yong (Department of Life Science, Iksan National College)
  • 곽준수 (마산대학교 전통약재개발과) ;
  • 류승희 (인제대학교 식품과학부) ;
  • 문갑순 (인제대학교 식품과학부) ;
  • 최동성 (우석대학교 생명공학부) ;
  • 박성혜 (원광대학교 한의학 전문대학원) ;
  • 한종현 (원광대학교 한의학 전문대학원) ;
  • 안병용 (익산대학 생명과학과)
  • Published : 2002.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Lycii Cordex Radicis extract (gigolpi) examined through SOS Chromotest showed a strong, dose-dependent antimutagenic effect on the tert-butyl hydroperoxide (t-BOOH) induced mutagenecity. Gigolpi revealed considerable superoxide anion radical scavenging activity under L-ascorbic $acid-CuSO_4$ system, but showed lower hydroxyl radical scavenging activity in photochemical test system. Hot-water gigolpi extract delayed protein oxidation, whereas lipid peroxidation of rat skin exposed to UVB radiation was inhibited. The results indicate that gigolpi possessing antioxidant activity against UVB-induced lipid peroxidation could be used as a raw ingredient for manufacturing functional cosmetics

본 연구는 지골피 물추출물을 이용하여 기능성 화장품을 개발하고자 생리기능(항돌연변이성, 라디칼 소거능 및 UV B 스트레스에 대한 항산화능)을 조사하였다. 지골피 물 추출물을 이용하여 tert-butyl hydroperoxide (t-BOOH)의 돌연변이원성에 대한 항돌연변이원성을, L-ascorbic acid-$CuSO_4$ 및 광화학적 시스템에서 수퍼옥사이드와 하이드록시 라디칼 소거능을 조사하였고, 비타민 E 결핍 쥐의 표피를 취하여 자외선 B조사로 산화적 스트레스를 유발시킨 뒤 지골피 물 추출물의 지방 및 단백질 산화 억제 효과를 조사하였다. SOS Chromotest에서 지골피 물 추출물은 t-BOOH의 변이원성에 대하여 강한 억제효과를 나타내었으며 억제 효과는 용량 의존적이었다. 광화학적 시스템에서 수퍼옥사이드의 라다칼 소거능은 비교적 강한 상쇄 효과를 나타내었지만 L-ascorbic acid-$CuSO_4$에서 하이드록시 라디칼 소거능은 낮았다. 자외선 B를 조사한 쥐 표피의 지질 과산화는 현저하게 억제되었으나 단백질 산화의 억제 효과는 나타나지 않았다. 이러한 결과는 UV B 방사시 지질과산화에 대해 항산화 활성을 나타낸 지골피 추출물을 기능성 화장품의 조원료로서의 사용가능성을 시사해준다.

Keywords

References

  1. Cha, S. H., Jung, Y. D., Lee, S. C., Ahn, B. W., Kim, Y. R, and Lee, M. W. (1991) Oxidation of skin tissue proteins by ultraviolet-B iiradiation. Kor. J. Gerontol. 1, 82-86
  2. Weminghaus, K., Keydani, M., Bhawan, J., Margolis, R., Blumberg, J. B., and Gilchrest, B. A. (1994) Evaluation of the photoprotective effect of oral vitamin E supplementation. Arch. Dermatol. 130, 1257-1261 https://doi.org/10.1001/archderm.130.10.1257
  3. Bolognia, J. L., Berwick, M. and Fine, J. A. (1990) Complete follow-up and evaluation of a skin cancer screening in Connecticut. J. Am. Acad. Dermatol. 23, 1098-1106 https://doi.org/10.1016/0190-9622(90)70340-N
  4. Robinson, J. K. (1990) Behavior modification obtained by sun protection enducation coupled with removal of a skin cancer. Arch. Dermatol. 126, 477-482 https://doi.org/10.1001/archderm.126.4.477
  5. Sutherland, B. M., Blackette, A. D. and Feng, N. I. (1985) Photoreactivation and other ultraviolet/visible light effects on DNA in human skin. Ann. N.Y. Acad. Sci., 453, 73-79 https://doi.org/10.1111/j.1749-6632.1985.tb11799.x
  6. Kellogg, E. W. and Froidovich, I. (1975) Superoxide, hydrogen peroxide, and singlet oxygen in lipid peroxidation by a xanthine oxidase system. J. Biol. Chem. 250, 8812-8817
  7. Danno, K., Horio, T., Takigawa, M. and Imamura, S. (1984) Role of oxygen intermediates in UV-induced epidermal cell injury. J. Invest. Dermatol. 83, 166-172 https://doi.org/10.1111/1523-1747.ep12263506
  8. Offord, E. A., Gautier, J. C., Avand, 0., Scaletta, C., Runge, F, Kramer, K. and Applegate, L. A. (2002) Photoprotection potential of lycopene, $\beta$-carotene, vitamin E, vitamin C and camosic acid in UVA-irradiated humam skin Sbroblasts. Free Radical Biology Medicine 32, 1293-130 https://doi.org/10.1016/S0891-5849(02)00831-6
  9. Mach1in, L. J. and Bendich, A. (1987) Free radical tissue damage; Protective role of andoxidant nutrients. J. FASEB 1, 441-446 https://doi.org/10.1096/fasebj.1.6.3315807
  10. Costa, H., GaUego, S. M. and Tomaro, M. L. (2002) EIfect of UV-B radiadon on antioxidant defense system in sunflower cotyledons. Plant Science 162, 939-945 https://doi.org/10.1016/S0168-9452(02)00051-1
  11. Park, J. S., Park, J . H and Lee, B. C. (1998) Effect of extraction procedures on chemical composidon and physical properties of Lycii Cortex. Korean J. Medical Crop Sci. 6, 91-95
  12. Quillardet, P and Hofnung, M. (1985) The SOS Chromotest, a coloiimetric bactehal assay for genotoxins: procedures. Mutation Res. 147, 65-78 https://doi.org/10.1016/0165-1161(85)90020-2
  13. Ginnopolitis, C. N. and Ries, S. K. (1997) Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol. 59, 309-314 https://doi.org/10.1104/pp.59.2.309
  14. Liu, F. and Ng, T. B. (2000) Antioxidative and free radical scavenging acdvities of selected medicinal herbs. Life Sci. 66, 723-735
  15. Oliver, C. N., Ahn, B., Moennan, E. J., Goldstein, S. A. and Stadtman, E. R. (1987) Age-related changes in oxidized proteins. J. Biol. Chem. 262, 5483-5491
  16. Ohkawa, H., Ohishi, N. and Yagi, K. (1979) Assay for lipid peroxides in animal tissues by thiobarbitunc acid reaction. Anal. Biochem. 95, 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  17. Stadler, R. H., Turesky, R. J., Muller, 0., Markovic, J. and Leong-Morgenthaler, P. M. (1994) The inhibitory effect of coffee on radical-mediated oxidadon and mutagenicity. Mutation Res. 308, 177-190 https://doi.org/10.1016/0027-5107(94)90153-8
  18. Davies, K. J. A. and Goigberg A. L. (1987) Protein damaged by oxygen radicals are rapidly degraded in extracts of red blood cells. J. Biol. Chem. 262, 8227-8234
  19. Miyaki, I. S. and Niwa, Y. (1987) Decreased skin superoxide dismutase activity by a single exposure of ultraviolet radiation is reduced by liposomal superoxide dismutase pretreatment. J.Inves. Dermatol. 89, 111-112
  20. Sugiura, K., Ueda, H., Jiraho, K., and Adachi, T. (1985) Studies on superoxide dismutase in human skin. Jpn J. Dermatol. 95, 1541-1545
  21. Fuchs, J., Huflejt, M. E., Rothfuss, L. M., Wilson, D. S., Carcamo, G., and Packer, L. (1989) Impairment of enzymic and nonenzymic antioxidants in skin by UVB irradiation. J. Inves. Dennatol. 93, 769-773 https://doi.org/10.1111/1523-1747.ep12284412
  22. Bissett, D. L., Chatterjee, R., Hannon, D. P. (1990) Photoprotective effect of superoxide scavenging antioxidants against ultraviolet radiation-induced chronic skin damage in the hairless mouse. Photodermatol Photoimmunol. Photomed. 1,56-62
  23. Darr, D., Cbmbs, S., Dunston, S., Manning, T., and Pinnell, S. (1992) Topical vitamin C protects porcine skin from ultraviolet radiation-induced damage. The British J. of Dennatol. 127,247-253