Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Primary Culture of Human Nasal Epithelial Cell Monolayer for In Vitro Drug Transport Studies

약물의 in vitro 투과 실험을 위한 사람의 비강점막상피세포 단층막의 일차배양

  • Yoo, Jin-Wook (College of Pharmacy, Pusan National University) ;
  • Kim, Yoo-Sun (College of Medicine, Pusan National University) ;
  • Lee, Min-Ki (College of Medicine, Pusan National University) ;
  • Roh, Hwan-Jung (College of Medicine, Pusan National University) ;
  • Lee, Chi-Ho (College of Pharmacy, Pusan National University) ;
  • Kim, Dae-Duk (College of Pharmacy, Pusan National University)
  • Published : 2002.03.20
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Abstract

The primary culture of human nasal epithelial cell monolayer was performed on a Transwell. The effect of various factors on the tight junction formation was observed in order to develop an in vitro experimental system for nasal transport studies. Human nasal epithelial cells, collected from human normal inferior turbinates, were plated onto diverse inserts. After 4 days, media of the apical surface was removed for air-liquid interface (ALI) culture. Morphological characteristics was observed by transmission electron microscopy (TEM). A polyester membrane of $0.4\;{\mu}m$ pore size was determined as the most effective insert based on the change in the transepithelial electric resistance (TEER) value as well as the $^{14}C-mannitol$ transport study. The ALI method was effective in developing the tight junction as observed in the further increase in the TEER value and reduction in the permeability coefficient $(P_{app})$ of $^{14}C-mannitol$ transport. Results of the transport study of a model drug, budesonide, showed that the primary culture system developed in this study could be further developed and applied for in vitro nasal transport studies.

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