Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Immuno-regulatory Property of Fruit-Extracts of Cornus kousa Burg.

산딸나무열매 추출물의 면역조절기능

  • 김종석 (우석대학교 생명자원과학부) ;
  • 오찬호 (우석대학교 생명공학부) ;
  • 전훈 (우석대학교 한약학과) ;
  • 이기승 (우석대학교 화학환경화공학부) ;
  • 마상용 (우석대학교 생명자원과학부)
  • Published : 2002.12.31
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Abstract

This study was conducted to investigate the immuno-regulatory effect and apoptosis of L1210 and HL60 leukemia cells of methanol-extracts of Cornus kousa Burg(CKB). The proliferation of mouse splenocytes and thymocytes enhanced by the addition of $10\;{\mu}g/ml$ of CKB. CKB were administered p.o. once a day for 7 days in adult male BALB/c mice. CKB increased the splenic and thymic T lymphocytes, especially the number of $T_H$ cells markedly increased by the treatment of CKB. CKB treatment induced the apoptotic cell death in L1210 mouse leukemia and HL60 human leukemia cells. In addition, CKB also accelerated the phagocytic activity in peritoneal macrophages and increased the production of plaque forming cells. These results suggest that CKB have an various immuno-regulatory property.

Keywords

References

  1. 肅培根. 1994. 중국본초도감 제 2권, p. 148. 동국대학교 한의대 본초학회 번역. 여강출판사
  2. Roitt I, Brostoff J, Male D. 1998. Immunology. 4th edition. Mosby, LondonMosmann T. 1983. Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxic assays. J. Immunol. methods 65 : 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  3. Suda T, Nagata S. 1994. Purification and characterization of the Fas-iigand that induces apoptosis. j. Exp. Med. 179: 873-879 https://doi.org/10.1084/jem.179.3.873
  4. Dowdy S, Weardon S. 1983. Statistics for research. p. 262. Wiley, New York
  5. Nicoletti I, Migliorati G, Pagliacci MC, Grignani G, Riccardi CA 1991. Rapid and simple method for measuring thymocyte apoptosis by propidiurn iodide staining and flow cytometry. J. Immunol. Methods 139 : 271-279 https://doi.org/10.1016/0022-1759(91)90198-O
  6. Miceli MC, Parnes JR. 1993. The role of CD4 and CD8 in T cell activation and differentiation. Advances in Immunology 53 : 59-64 https://doi.org/10.1016/S0065-2776(08)60498-8
  7. Blair AL, Cree IA, Beck IS, Hating MIG. 1988. Measurement of phagocyte chemiluminescence in a microtiter plate format. J Immunol. Methods 112: 163-168 https://doi.org/10.1016/0022-1759(88)90352-3
  8. Cunningham AI, Szenberg A. 1968. Further improvements in the plaque technique for detecting single antibody forming cells. Immunology 14 : 559-564
  9. Kerr JFR, Wyllie AH, Currie AR. 1972. Apoptosis. a basic biological phenomenon with wider implications in tissue kinetics. Br. J. Cancer 26 : 239-245 https://doi.org/10.1038/bjc.1972.33
  10. Nagata S. 1997. Apoptosis by death factor. Cell 88 : 355-365 https://doi.org/10.1016/S0092-8674(00)81874-7
  11. Dive C, Gregory CD, Phipps DJ, Evans DL, Milner AE, Wyllie AH. 1992. Analysis and discrimination of necrosis and apoptosis(programmed cell death) by multiparameter flow cytometry. Biochimica et Biophysica Acta 1133 : 275-285 https://doi.org/10.1016/0167-4889(92)90048-G
  12. D'Amico AV, McKenna WG. 1994. Apoptosis and a reinvestigation of the biologic basis for cancer therapy. Radiotherapy & Oncology 33(1) : 3-10 https://doi.org/10.1016/0167-8140(94)90079-5
  13. Telford WG, King LE, Fraker PJ. 1991. Evaluation of glucocorticoid-induced DNA fragmentation in mouse thymocytes by flow cytometry. Cell Prolif. 24 : 447-459 https://doi.org/10.1111/j.1365-2184.1991.tb01173.x