생약학회지 (Korean Journal of Pharmacognosy)

한국생약학회 (The Korean Society of Pharmacognosy)
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자생식물 추출물의 세포접착인자 저해활성 검색

Inhibitory Effects of Natural Plant Extracts on ICAM-1/LFA-1 Mediated Adhesion of HL-60 Cells

  • 권오억 (한국생명공학연구원 지질대사연구실) ;
  • 이승웅 (한국생명공학연구원 지질대사연구실) ;
  • 정미연 (한국생명공학연구원 지질대사연구실) ;
  • 김영호 (충남대학교 약학대학) ;
  • 이현선 (한국생명공학연구원 지질대사연구실) ;
  • 김영국 (한국생명공학연구원 지질대사연구실) ;
  • 노문철 (한국생명공학연구원 지질대사연구실)
  • Kwon, Oh-Eok (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Seung-Woong (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Mi-Yeon (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University) ;
  • Lee, Hyun-Sun (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Kook (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Rho, Mun-Chual (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2002.12.30
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초록

Atherosclerosis is a progressive disease characterized by the accumulation of lipids and fibrous elements in the arteries. Monocyter/macrophages are involved in many aspects of the development of atherosclerotic plaques. It is known that the intercellular adhesion molecule-1(ICAM-1) expressed preferentially on endothelial cells of atherosclerotic plaque, promotes local adhesion and transendothelial migration of monocytes, neutrophils, and lymphocytes. Using the human promyelocytic leukemia HL-60 cell line, we investigated the inhibitory effects of methanol extracts of 175 natural plants on ICAM-1/LFA-1 mediated cell adhesion. Eight kinds of methanol extracts of tested plants inhibited PMA-induces homotypic aggregationof HL-60 cells without cytotoxicity at the concentration of $6.25\;{\mu}g/ml$. They were divided two fractions of $CHCI_3$ and $H_2O$ to use solvent partition. Among them, $CHCI_3$ extract $(1.0\;{\mu}g/ml)$ of Saururus chinensis and Chloranthus japonicus singificantly inhibited aggregation of HL-60 cells without cytotoxicity, respectively.

키워드

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