Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Screening of Monocyte Chemoattractant Protein-1-Induced Chemotaxis Inhibitors from Medicinal Herbs

생약자원으로부터 Monocyte Chemoattractant Protein-1에 의한 Chemotaxis 저해활성 검색

  • Lee, Seung-Woong (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Oh-Eok (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Mi-Yeon (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University) ;
  • Lee, Hyun-Sun (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Kook (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology) ;
  • Rho, Mun-Chual (Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology)
  • 이승웅 (한국생명공학연구원 지질대사 연구실) ;
  • 권오억 (한국생명공학연구원 지질대사 연구실) ;
  • 정미연 (한국생명공학연구원 지질대사 연구실) ;
  • 김영호 (충남대학교 약학대학) ;
  • 이현선 (한국생명공학연구원 지질대사 연구실) ;
  • 김영국 (한국생명공학연구원 지질대사 연구실) ;
  • 노문철 (한국생명공학연구원 지질대사 연구실)
  • Published : 2002.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Blood monocytes are the precursors for the lipid-laden foam cells of early atherosclerotic lesions. Monocyte chemoattractant protein-1(MCP-1), a CC chemokine, and chemokine receptor 2 (CCR2) play a crucial role in the recruitment of monocytes to the vascular lesion. Using the human monocyte THP-1 cell line, we investigate the inhibitory effects of methanol extracts of 127 medicinal herbs on MCP-1 induced chemotaxis. Seven kinds of methanol extracts of medicinal herbs showed above 40% inhibitory effect with the concentration of $25{\mu}g/ml$. They were divide three fractions of $CHCI_3$, BuOH, $H_2O$ to use solvent partition. Among them, butanol extract of Junci Medulla and $CHCI_3$ extract of Clematidis Radix are showed significant inhibitory activities (above 50% inhibition) at the same concentration.

Keywords

References

  1. Ross, R. (1995) Cell biology of atherosclerosis. Annu. Rev. Physiol. 57: 791-804 https://doi.org/10.1146/annurev.ph.57.030195.004043
  2. Gerszten, R. E., Lim, Y. C., Ding, H. T., Snapp, K., Kansas, G. Dichek, D. A., Cabanas, C., Francisco, S. M., Gimbrone Jr, M. A., Rosenzweig, A. and Lusinskas, F. W. (1998) Adhesion of Monocytes to Vascular Cell Adhesion Molecule-1-Transduced Human Endothelial Cells : Implications for Atherogenesis. Circ. Res. 82: 871-878 https://doi.org/10.1161/01.RES.82.8.871
  3. Power, C. A. and Proudfoot, A. E. (2001) The chemokine system: novel broad-spectrum therapeutic targets. Curr. Opin. Pharmacol. 1: 417-424 https://doi.org/10.1016/S1471-4892(01)00072-8
  4. Gosling, J., Slaymaker, S., Gu, L., Tseng, S., Zlot, C. H., Young, S. G., Rollins, B. J. and Charo, I. F. (1999) MCP-l deficiency reduces susceptibility to atheroslcerosis in mice that overexpress human apolipoprotein B. J. Ctin. Invest. 103: 773-778 https://doi.org/10.1172/JCI5624
  5. Baggiolini, M. and Loetscher, P. (2000) Chemokines in inflammation and immunity. Immunol. Today 21: 418-420 https://doi.org/10.1016/S0167-5699(00)01672-8
  6. Takeya, M., Yoshimura, T., Leonard, E. J. and Takahashi, K. (1993) Detection of monocyte chemoattractant protein-1 in human atherosclerotic lesions by an anti-monocyte chemoattractant protein-1 monoclonal antibody. Hum. Pathol. 5: 534-539
  7. Nelken, N. A., Coughlin, S. R., Gordon, D. and Wilcox, J. N. (1991) Monocyte chemoattractant protein-1 in human atheromatous plaques. J. Clin. Invest. 4: 1121-1127
  8. Seino, Y., Ikeda, U., Sekiguchi, H., Morita, M., Konishi, K., Kasahara, T. and Shimada, K. (1995) Expression of monocyte chemoattactant protien-l in vascularb tissue. Cytokine 6: 575-579
  9. Taub, D. D. (1996) Chemokine-leukocyte interactions. Cytokine Growth Factor Rev. 7: 355-376 https://doi.org/10.1016/S1359-6101(97)89237-4
  10. Harrington, J. R. (2000) The role of MCP-l in atherosclerosis. Stem Cells 18: 65-66 https://doi.org/10.1634/stemcells.18-1-65
  11. Gu, L., Okada, Y., Clinton, S. K., Gerard, C., sukhova, G. K., Libby, P. and Rollins, B. J. (1998) Absence of monocyte chemoattractant protein-1 reduces atheroslceorsis in low density lipoprotein receptor-deficient mice. Mol. Cett 2: 275-281
  12. Boring, L., Gosling, J., Cleary, M. and Charo, I. F. (1998) Decreased lesion formation in CCR2-/- mice reveals a role for chemokines in the initiation of atherosclerosis. Nature 394: 894-897 https://doi.org/10.1038/29788
  13. Tumer, S. J., Domin, J., Waterfield, M. D., Ward, S. G. and Westwick, J. (1998) The CC Chemokine Monocyte Chemoatactic Peptide-l Activates both the Class I p85/p110 Phosphatidylinositol 3-kinase and the Class II PI3K-C2. J. Bio. Chem. 273: 25987-25995 https://doi.org/10.1074/jbc.273.40.25987
  14. Horuk, R. and Ng, H. P. (2000) Chemokine receptor antagonists. Med. Res. Rev. 20: 155-168 https://doi.org/10.1002/(SICI)1098-1128(200003)20:2<155::AID-MED3>3.0.CO;2-G