사상체질의학회지 (Journal of Sasang Constitutional Medicine)

  • 제13권1호
  • /
  • Pages.61-69
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  • 2001
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  • 1226-4075(pISSN)
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  • 2287-786X(eISSN)
사상체질의학회 (The Society Of Sasang Constitutional Medicine)
권호 표지

인간뇌성상세포(人間腦星狀細胞)에서 열다한소탕(熱多寒少湯)에 의한 세포활성물질(細胞活性物質) 생성(生成) 조절(調節)에 관(關)한 연구(硏究)

Studies on the Cytokine Production Regulation in Human Astrocytes by Yuldahansotang

  • 최지숙 (원광대학교한의과대학 사상체질과) ;
  • 김경요 (원광대학교한의과대학 사상체질과) ;
  • 김형민 (원광대학교 한의과대학) ;
  • 주종천 (원광대학교한의과대학 사상체질과)
  • Choi, Ji-sook (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Kim, Kyoung-yo (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Kim, Hyoung-min (College of Oriental Medicine, Wonkwang Univ.) ;
  • Ju, Jong-chon (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.)
  • 발행 : 2000.12.31
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초록

사상의학적 견지에서 태음인(太陰人)의 중풍, 치매와 같은 신경계질환에 다용되고 있는 열다한소탕(熱多寒少湯)은 최근에 그 임상적 효과를 뒷받침할 다각적인 연구들이 이루어지고 있음에도 불구하고 그 정확한 약리학적 기전에 대해서는 밝혀지지 않고 있다. 본 연구에서는 인간성상세포를 이용하여 열다한소탕(熱多寒少湯)이 substance P (SP)와 lipopolysaccharide (LPS)에 의해 유도되는 다양한 세포활성물질의 분비량의 조절을 검토함으로써 열다한소탕(熱多寒少湯)의 약리기전을 면역학적 측면에서 보다 세밀하게 살펴보고자 하였다. 열다한소탕(熱多寒少湯) 수침액은 인간 뇌 성상세포로 부터 LPS와 SP의 동시자극에 의해 생성되는 세포활성물질중 interleukin (IL)-1, IL-4, IL-6 및 tumor necrosisfaccor-${\alpha}$ (TNF-${\alpha}$)의 분비를 농도의존적으로 억제했다. 그러나 interferon-${\gamma}$ (IFN-${\gamma}$) 및 IL-2의 분비 조절에는 영향을 미치지 않았다. 그리고 항 IL-$1{\beta}$ 항체에 의해 SP 유도성 TNF-${\alpha}$ 분비의 증가가 억제되기 때문에 IL-1은 TNF-${\alpha}$ 증가를 매개하는 역할을 하는 것으로 사료된다. 이상의 결과는 열다한소탕(熱多寒少湯)에 의한 급성기 중풍환자 치료 효과가 뇌 성상세포로부터 분비되는 세포활성물질의 조절과 밀접한 관련성이 있다는 것을 암시하고 있다.

키워드

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