IMMUNE NETWORK
- Volume 1 Issue 1
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- Pages.61-69
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- 2001
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
Generation and maintenance of type II collagen-specific T-cell line expressing conserved TCR-CDR3 motifs among patients with rheumatoid arthritis
류마티스 관절염 환자에서 Conserved T 세포 수용체의 CDR3 motif를 표현하는 제2형 콜라겐 특이 T세포주의 형성과 유지
- Kim, Seung-Hoon (Catholic Institute of Immunobiology, Catholic University of Korea) ;
- Cho, Mi-La (Catholic Institute of Immunobiology, Catholic University of Korea) ;
- Youn, Jeehee (Department of Anatomy, Hanyang University Medical School) ;
- Park, Sung-Hwan (Center of Rheumatic Disease, Kangnam St. Mary's Hospital, Catholic University of Korea) ;
- Hwang, Sue-Yun (Center of Rheumatic Disease, Kangnam St. Mary's Hospital, Catholic University of Korea) ;
- Cho, Chul-Soo (Catholic Institute of Immunobiology, Catholic University of Korea) ;
- Kim, Ho-Youn (Catholic Institute of Immunobiology, Catholic University of Korea)
- 김승훈 (가톨릭대학교 의과대학 의과학원 면역생물연구소) ;
- 조미라 (가톨릭대학교 의과대학 의과학원 면역생물연구소) ;
- 윤지희 (한양대학교 의과대학 해부학교실) ;
- 박성환 (가톨릭대학교 의과대학 강남성모병원 류마티스 센터) ;
- 조철수 (가톨릭대학교 의과대학 강남성모병원 류마티스 센터) ;
- 황수연 (가톨릭대학교 의과대학 의과학원 면역생물연구소) ;
- 김호연 (가톨릭대학교 의과대학 의과학원 면역생물연구소)
- Published : 2001.04.30
Abstract
Background: To determine the molecular structure of type II collagen-specific T-cell receptors associated with rheumatoid arthritis (RA). Methods: We generated CII-specific T-cell lines of 8 RA patients by prolonged in vitro culture with bovine CII (bCII) and the immunogenic peptide (256-270) of human CII. The proliferation response towards CII stimulation was measured from the uptake of 3H-thymidine. Changes in the secretion of Th 1 and Th2 cytokines in the culture supernatent were measured by ELISA. The TCR clonotypes of these T-cells were examined by RT-PCR/SSCP analyses of all 22