Journal of Korean Neurosurgical Society
- 제30권sup1호
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- Pages.13-19
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- 2001
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- 2005-3711(pISSN)
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- 1598-7876(eISSN)
아데노 바이러스 Cytosine Deaminase/Thymidine Kinase 융합 유전자의 항 종양효과
Antitumor Effect of an Adenoviral Cytosine Deaminase/Thymidine Kinase Fusion Gene in C6 Glioma Cells
- 김영우 (연세대학교 의과대학 신경외과학교실) ;
- 최재영 (연세대학교 의과대학 신경외과학교실) ;
- 장진우 (연세대학교 의과대학 신경외과학교실) ;
- 박용구 (연세대학교 의과대학 신경외과학교실) ;
- 정상섭 (연세대학교 의과대학 신경외과학교실)
- Kim, Young Woo (Department of Neurosurgery, Yonsei University College of Medicine) ;
- Choi, Jae Young (Department of Neurosurgery, Yonsei University College of Medicine) ;
- Chang, Jin Woo (Department of Neurosurgery, Yonsei University College of Medicine) ;
- Park, Yong Gou (Department of Neurosurgery, Yonsei University College of Medicine) ;
- Chung, Sang Sup (Department of Neurosurgery, Yonsei University College of Medicine)
- 투고 : 2001.07.01
- 심사 : 2001.10.08
- 발행 : 2001.12.31
초록
Objective : We investigated the feasibility of a double suicide gene/prodrug therapy, involving direct introduction of the herpes simplex virus Type 1 thymidine kinase(TK) gene and the Escherichia coli cytosine deaminase(CD) gene, via a recombinant adenoviral vector and ganciclovir(GCV) and/or 5-fluorocytosine(5-FC) treatment, in C6 glioma cells. Methods : Efficient gene transfer and transduction of C6 glioma cells via a recombinant adenovirus were evaluated by infecting cells with adenovirus bearing the