Journal of Korean Neurosurgical Society

대한신경외과학회 (The Korean Neurosurgical Society)
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사람 교모세포종 A172 세포에서 Cisplatin에 의한 세포고사의 유도

Apoptosis Induced by Cisplatin in Human Glioblastoma A172 Cells

  • 김대원 (원광대학교 의과대학 신경외과학교실) ;
  • 김태영 (원광대학교 의과대학 신경외과학교실) ;
  • 박종태 (원광대학교 의과대학 신경외과학교실) ;
  • 김종문 (원광대학교 의과대학 신경외과학교실)
  • Kim, Dae Won (Department of Neurosurgery, School of Medicine, Wonkwang University) ;
  • Kim, Tae Young (Department of Neurosurgery, School of Medicine, Wonkwang University) ;
  • Park, Jong Tae (Department of Neurosurgery, School of Medicine, Wonkwang University) ;
  • Kim, Jong Moon (Department of Neurosurgery, School of Medicine, Wonkwang University)
  • 투고 : 2001.06.19
  • 심사 : 2001.12.03
  • 발행 : 2001.12.31
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초록

This study was designed to investigate the mechanism of cell death after cisplatin treatment in human glioblastoma A172 cells. Cis-diamminedichloroplatinum(Cisplatin) demonstrated cytostatic or cytotoxic effects on A172 cells in a dosedependent manner. Cisplatin-mediated cytotoxity in A172 cells was revealed as an apoptosis characterized by high molecular weight DNA fragmentation by agarose electrophoresis as well as nuclear fragmentation by Hoechst staining. Cisplatin also resulted in the activation of caspase 3-like protease as well as poly(ADP-ribose) polymerase(PARP) cleavage. Interestingly, the anti-apoptotic Bcl2 protein was degraded and furthermore, expression of p53 protein was increased by cisplatin in a time-dependent manner. Taken together, these results suggest that anticancer drug, cisplatin induces the apoptotic death of human glioblastoma A172 cells via the activations of caspase 3-like protease, degradation of anti-apoptotic Bcl2 protein and increase in the expression of p53.

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