Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Benefical Effect of Cordyceps Sinensis Sacc. Extract (CSS) on Oxidant-Induced Membrane Tpransport Dysfunction in Rabbit Renal Cortical Slices

동충하초약침액(冬蟲夏草藥鍼液)이 가토(家兎) 신피질절편(腎皮質切片)에서 세포막물질이동계(細胞膜物質移動系)의 기능장애(機能障碍)에 미치는 영향(影響)

  • Cheon, Kap-Sool (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Seo, Jung-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Youn, Hyoun-Min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-Ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Ahn, Chang-Beohm (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Jang, Kyung-Jeon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University)
  • 천갑술 (동의대학교 한의과대학 침구경혈학교실) ;
  • 서정철 (동의대학교 한의과대학 침구경혈학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실)
  • Received : 2001.05.07
  • Accepted : 2001.05.19
  • Published : 2001.06.20
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Abstract

Objective : This study was undertaken to determine whether Cordyceps sinensis Sacc. (CSS) extract exerts the protective effect against oxidant-induced alterations in membrane transport function in renal tubules. Methods : Membrane transport fucntion was estimated by examining alterations in p-aminohippurate (PAH) uptake in rabbit renal cortical slices. For induction oxidative stress, slices were treated with an organic peroxide cumene hydroperoxide for 60 min at $37^{\circ}C$. Cumene hydroperoxide inhibited PAH uptake in a time dependent manner. Results : CSS at 0.5-5% concentrations prevented cumene hydroperoxide-induced inhibition of PAH uptake. CSS at 1% also attenuated LDH release and lipid peroxidation induced by cumene hydroperoxide. When slices were treated with 0.2 mM mercury chloride, PAH uptake was inhibited and lipid peroxidation was increased. These changes by mercury were significantly prevented by CSS. Conclusion : These results suggest that CSS prevents oxidant-induced alterations in membrane transport function in rabbit renal cortical slices. Such protective effect of CSS may be attributed to inhibition of peroxidation of membrane lipid.

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