대한수의학회지 (Korean Journal of Veterinary Research)
- 제41권3호
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- Pages.333-342
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- 2001
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Infectious bursal disease 백신주의 VP2 gene의 hypervariable region 분석
Sequence analysis of the hypervariable region in VP2 gene of infectious bursal disease vaccine strains
- Park, Yoo-jin (Kangwon provincial chunchon hunting grounds) ;
- Kim, Soo-joung (Department of Veterinary Medicine, Kangwon National University) ;
- Kwon, Hyuk-moo (Department of Veterinary Medicine, Kangwon National University)
- 심사 : 2001.06.27
- 발행 : 2001.09.25
초록
To detect the genetic variations among infectious bursal disease (IBD) vaccine strains, the hypervariable region of VP2 gene of seven IBDV vaccine strains were amplified using reverse transcriptase/polymerase chain reation(RT/PCR). Ampllified PCR products of IBDV were cloned, sequenced, and compared with published sequences for IBDV. Vaccine strains (JOONG, HAN, B7, IB, BU2, G2, CIL) used in Korea and Korean field isolates (SH/92, K1, 310) had 81%(310 and HAN) ~ 98%(SH/92 and CIL) amino acid sequence similarity. Vaccine strains had 80%(HAN and IB) ~ 99%(JOONG and BU2) amino acid sequence similartiy. Intermediate plus vaccine strain, CIL was not substituted at positions 279(D