Asian-Australasian Journal of Animal Sciences
- 제14권12호
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- Pages.1781-1784
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- 2001
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- 1011-2367(pISSN)
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- 1976-5517(eISSN)
DOI QR Code
Influence of Antisense IGFBP-2 Oligo Deoxynucleotide Administration on Tissue IGFBP-2 Gene Expression in Chicks
- Nagao, K. (Laboratory of Grassland Science, University Farm, Graduate School of Bioagricultural Sciences, Nagoya University) ;
- Osada, K. (Laboratory of Animal Nutrition, Graduate School of Bioagricultural Sciences, Nagoya University) ;
- Murai, A. (Laboratory of Animal Nutrition, Graduate School of Bioagricultural Sciences, Nagoya University) ;
- Okumura, J. (Laboratory of Animal Nutrition, Graduate School of Bioagricultural Sciences, Nagoya University) ;
- Kita, K. (Laboratory of Grassland Science, University Farm, Graduate School of Bioagricultural Sciences, Nagoya University)
- 투고 : 2001.01.02
- 심사 : 2001.06.23
- 발행 : 2001.12.01
초록
We have examined the influence of antisense oligo deoxynucleotide (ODN) of IGFBP-2 on tissue IGFBP-2 gene expression in chicks. Antisense IGFBP-2 ODN was directly injected into the liver or cerebroventricle. Control birds were injected with vehicle. The hepatic IGFBP-2 gene expression was decreased to approximately 30% of the control at 2 h after injection of antisense ODN. In the brain of chickens injected with antisense ODN, IGFBP-2 mRNA level did not change after 2 h of injection and decreased to approximately 60% of the control after 6 h of injection. These results showed that the expression of IGFBP-2 gene in the liver and brain was successfully suppressed by administrating antisense ODN and that hepatic IGFBP-2 gene expression was quickly suppressed by antisense ODN compared with the brain.