Journal of Periodontal and Implant Science
- Volume 31 Issue 1
- /
- Pages.135-148
- /
- 2001
- /
- 2093-2278(pISSN)
- /
- 2093-2286(eISSN)
Effects of Replicative Senescence on the Cell Cycle Regulation in Human Gingival Fibroblasts
치은섬유아세포의 복제노화가 세포주기 조절에 미치는 영향
- Park, Young-Chae (Department of Periodontology, College of Dentistry, Wonkwang University) ;
- Yang, Dae-Seung (Department of Periodontology, College of Dentistry, Wonkwang University) ;
- Kim, Jae-Ho (Department of Periodontology, College of Dentistry, Wonkwang University) ;
- Kim, Hyun-A (Department of Periodontology, College of Dentistry, Wonkwang University) ;
-
You, Yong-Ouk
(Department of Oral Biochemistry, College of Dentistry, Wonkwang University) ;
-
Sin, Hyung-Shik
(Department of Periodontology, College of Dentistry, Wonkwang University)
- 박영채 (원광대학교 치과대학 치주과학교실) ;
- 양대승 (원광대학교 치과대학 치주과학교실) ;
- 김재호 (원광대학교 치과대학 치주과학교실) ;
- 김현아 (원광대학교 치과대학 치주과학교실) ;
-
유용욱
(원광대학교 치과대학 구강생화학교실) ;
-
신형식
(원광대학교 치과대학 치주과학교실)
- Published : 2001.03.30
Abstract
Gingival fibroblasts are major cellular component of gingiva. However, the molecular mechanisms of senescence of human gingival fibroblasts are unknown. Human fibroblasts undergo replicative senescence in vitro after a limited number of population doublings. A reduced rate of proliferation is a prominent phenomenon observed in senescent fibroblasts. This phenomenon is controled by cell cycle regulatory proteins. The purpose of present study was to investigate the effect of replicative senescence on cell cycle progression and to find out its molecular mechanisms in human gingival fibroblasts. Replicative senescence of gingival fibroblasts were induced by subsequent cultures that were repeated up to 18 passage. In the present study, I examined change of cell proliferation, cell activity, cell viability and cell cycle progression during the replicative process. Also, I examined expression of cell cycle regulatory proteins which was estimated by western blot analysis. Cell proliferation, cell activity and cell viability of gingival fibroblasts were notably decreased with increase of population doubling level(PDL). S phase was decreased and G1 phase was increased with increase of PDL. Western blot analysis showed that levels of P16, p21 and p53 of senescent gingival fibroblasts(PDL41, PDL58) were higher than young fibroblasts(PDL27) and cdk4 were lower than young fibroblasts(PDL27). In conclusion, these results suggest that proliferative function of human gingival fibroblasts may be decreased by replicative senescence and its molecular mechanisms may be activatied with p16, p21, p53 and pRB, and repressed wtih cdk4.
Keywords