Abstract
The concentration of free PAs in the cells was decreased gradually from $500{\mu}mole$ to $290{\mu}mole$ for PUT, from $153{\mu}mole$ to $79{\mu}mole$ for SPD, from $69{\mu}mole$ to $20{\mu}mole$ for SPM during the ABA treatment within 1 hour, and was slightly changed with the increasing or decreasing between $290{\mu}mole{\sim}220{\mu}mole$ in the constant content level of PUT during the continuously ABA treatment of 2 days in the young cotyledons. The concentration of free PUT was gradually decreased from $160{\mu}mole$ to $9{\mu}mole$ during the ABA treatment within 1 hour like as cotyledons, and decreased from $9{\mu}mole$ to $5{\mu}mole$ during the continuously ABA treatment 2 days in the young hypocotyls. PUT and other PA were existed $5{\mu}mole$ during the continuously ABA treatment of 2 days in the hypocotyls. It showed that during the ABA treatment was decreased all PA concentration in the cells and PAs were not concerned directly to stress, but might regulated physiological change against stress.
자엽에 ABA를 처리하면 세포내에 있는 유리 PA은 1시간 이내에 $500{\mu}mole$에서 $290{\mu}mole$로, SPD는 $153{\mu}mole$에서 $79{\mu}mole$로, SPM은 $69{\mu}mole$에서 $20{\mu}mole$로 점진적으로 감소되었다. 2일동안 지속적으로 ABA를 처리하면 PUT는 $290{\mu}mole{\sim}234{\mu}mole$ 사이의 일정한 수준을 유지하면서 증감의 변화만을 보였다. 하배축에다 ABA를 처리하면 자엽에서와 같이 유리 PUT는 1시간 이내에 $160{\mu}mole$에서 $9{\mu}mole$까지 점진적으로 감소되었다. 2일동안 지속적인 ABA 처리동안 하배축에 있는 유리 PUT과 다른 PA들은 $5{\mu}mole$ 정도로 존재하였다. ABA처리는 생체내에 존재하는 유리 PA의 농도를 감소시켜 직접적으로 스트레스에 관여하는 것이 아니라 스트레스에 대항하여 생체가 미리 준비하도록 생리 및 생화학적인 변화를 조절하는 것으로 보인다.