Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지

PCR-Based Sensitive Detection of Wood-Decaying Fungus Phellinus linteus by Specific Primer from rDNA ITS Regions

  • Park, Dong-Suk (Molecular Genetics Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kang, Hee-Wan (Molecular Genetics Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Ki-Tae (Seolin Bioscience Institute) ;
  • Cho, Soo-Muk (Ja-Kwang Research Institute) ;
  • Park, Young-Jin (Molecular Genetics Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Shin, Hye-Sun (Molecular Genetics Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Byoung-Moo (Molecular Genetics Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Go, Seung-Joo (Molecular Genetics Division, National Institute of Agricultural Science and Technology, RDA)
  • Published : 2001.03.31
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Abstract

Based on the rDNA ITS sequences data, specific primer set for PCR detection of wood-decaying fungus Phellinus linteus was designed. The length of PCR products using designed primer set(SHF and SHR) was about 540 bp. Among 11 species, 17 isolates of Phellinus spp. including Phellinus linteus, P. pomaceus, P. spiculosus, P. baumi, P. pini, P. igniarius, P. gilvus, P. biscuspidatus, P. weirii, P. johnsonianus, P. robutus, and P. igniarius, seven isolates of Phellinus linteus showed about 540 bp-sized single band. This molecular technique could offer a useful tool for detecting and identifying Phellinus linteus.

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