The Effects of Artemisia capillaries Herbal Acupuncture on Ethanol-induced Apoptosis in Neuroblastoma Cell Line

인진 약침액이 신경아세포주에서 에탄올에 의해 유발된 아폽토시스에 미치는 영향

  • Ee-Hwa, Kim (College of Oriental Medicine, Semyung University) ;
  • Youn-Hee, Kim (Departments of Meridianology and Chemistry, Semyung University) ;
  • Youn-Jung, Kim (Departments of Physiology, College of Medicine, Kyunghee University) ;
  • Mi-Hyun, Jang (Departments of Physiology, College of Medicine, Kyunghee University) ;
  • Joo-Ho, Chung (Departments of Pharmacology, College of Medicine, Kyunghee University) ;
  • Chang-Ju, Kim (Departments of Physiology, College of Medicine, Kyunghee University)
  • Published : 2001.03.01

Abstract

목적:인진 약침액이 SK-N-MC 신경아세포주에서 에탄올에 의해 유발된 아폽토시스에 미치는 영향을 조사하였다. 방법 : SK-N-MC cell line에서의 아폽토시스 변화를 관찰하기 위해서 MTT assay, DAPI staining 및 flow cytometric analysis 방법을 이용하였다. 결과: MTT assay를 이용하여 분석한 결과 농도에 따른 세포 독성의 효과가 에탄올 투여로부터 관찰되었다. 또한 인진 약침액으로 전처치하고 에탄올을 처치하였을 때 세포 독성이 크게 감소되었다. DAPI staining에서 인진 약침액 투여군은 에탄올 투여군에 비해서 fragmentation이 억제되었다. Flow cytometry를 통하여 인진 약침액 투여군은 에탄올 투여군에 비하여 세포주기 중 sub $G_1$ 분획의 증가가 억제되었다. 결론 : SK-N-MC 신경아세포주에서 에탄올에 의해서 유발된 아폽토시스는 전형적인 세포사별 형태를 나타내었다. 또한 인진 약침액은 에탄올에 의해서 유발된 아폽토시스에서 세포보호 효과가 있음이 확인되었다.

Keywords

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