한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)
- 제46권2호
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- Pages.112-120
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- 2001
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- 0252-9777(pISSN)
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- 2287-8432(eISSN)
Genetic Diversity Based on Morphology and RAPD Analysis in Vegetable Soybean
- Srinives, P. (Department of Agronomy, Kasetsart University, Kamphaeng Saen) ;
- Chowdhury, A.K. (University of Science and Technology, Patuakhali) ;
- Tongpamnak, P. (Central Laboratory and Greenhouse Complex. Kasetsart University) ;
- Saksoong, P. (Department of Genetics, Faculty of Science, Kasetsart University)
- 발행 : 2001.06.01
초록
Genetic diversity of 47 East-Asian vegetable soybean was characterized by means of agro-morphological traits and RAPD markers. A field trial was conducted to evaluate 14 agro-morphological traits. To study RAPD-based DNA analysis, a total of sixty 10-mer random primers were screened. Of these, 23 polymorphic markers in 16 varieties used for screening. Among 207 markers amplified, 48 were polymorphic for at least one pairwise comparison within the 47 varieties. A higher differentiation level between varieties was observed by using RAPD markers compared to morphological markers. Correspondence analysis using both types of marker showed that RAPD data could fully discriminate between all varieties, whereas morphological markers could not achieve a complete discrimination. Genetic distances between the varieties were estimated from simple matching coefficients, ranged from 0.0 to 0.640 with an average of 0.295