한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)
- 제46권2호
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- Pages.106-111
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- 2001
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- 0252-9777(pISSN)
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- 2287-8432(eISSN)
Determination of Protein and Oil Contents in Soybean Seed by Near Infrared Reflectance Spectroscopy
- Choung, Myoung-Gun (National Yeongnam Agricultural Experiment Station, RDA) ;
- Baek, In-Youl (National Yeongnam Agricultural Experiment Station, RDA) ;
- Kang, Sung-Taeg (National Yeongnam Agricultural Experiment Station, RDA) ;
- Han, Won-Young (National Yeongnam Agricultural Experiment Station, RDA) ;
- Shin, Doo-Chull (National Yeongnam Agricultural Experiment Station, RDA) ;
- Moon, Huhn-Pal (National Yeongnam Agricultural Experiment Station, RDA) ;
- Kang, Kwang-Hee (College of Natural Resources, Yeungnam University)
- 발행 : 2001.06.01
초록
The applicability of near infrared reflectance spectroscopy(NIRS) was tested to determine the protein and oil contents in ground soybean [Glycine max (L.) Merr.] seeds. A total of 189 soybean calibration samples and 103 validation samples were used for NIRS equation development and validation, respectively. In the NIRS equation of protein, the most accurate equation was obtained at 2, 8, 6, 1(2nd derivative, 8 nm gap, 6 points smoothing and 1 point second smoothing) math treatment condition with SNV-D (Standard Normal Variate and Detrend) scatter correction method and entire spectrum by using MPLS (Modified Partial Least Squares) regression. In the case of oil, the best equation was obtained at 1, 4, 4, 1 condition with SNV-D scatter correction method and near infrared (1100-2500nm) region by using MPLS regression. Validation of these NIRS equations showed very low bias (protein:-0.016%, oil : -0.011 %) and standard error of prediction (SEP, protein: 0.437%, oil: 0.377%) and very high coefficient of determination (