Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Cytological Analysis of Microspores during Temperature Pretreatment in Anther Culture of Capcicum annuum L.

고추의 약배양 시 온도 전처리에 따른 소포자의 세포학적 변화 분석

  • 김문자 (목원대학교 자연과학대학 생명과학과) ;
  • 장인창 (목원대학교 자연과학대학 생명과학과)
  • Published : 2001.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Inoculated anthers of Capsicum annuum L. were subjected to 4 and 32$^{\circ}C$ pretreatment and their influence on the microspore viability, early cytological changes and the induction frequency of microspore embryo was investigated. Viability of freshly isolated microspores was between 62 and 64%. During temperature pretreatment, microspore viability showed a rapid decrease and this tendency enhanced with the 32$^{\circ}C$ pretreatment. Irrespective of temperature pretreatment, microspore viability declined to nearly zero after nine days. Before temperature pretreatment, most of the microspores in anthers were at late uninucleate stage. Several types of multinuclear microspores appeared from the 2 day after culture onwards, together with many degenerated and non-induced microspores. The 32$^{\circ}C$ pretreatment gave higher proportions of embryogenic microspore than other treatment. However, the temperature pretreatment had no clear effect on the frequencies of symmetrical binucleate rnicrospore. The multinucleate grains might originate either by symmetrical or asymmetrical division. After 2 days of pretreatment at 25 and 32$^{\circ}C$ , degenerated microspore increased above 50%. In contrast, during 4$^{\circ}C$ treatment, nucleus of most microspores remained intact for 14 days. The 32$^{\circ}C$ pretreatment produced more embryos than 4$^{\circ}C$ treatment. The most effective period of 32$^{\circ}C$ pretreatment was 4 days. In contrast, effective period of 4$^{\circ}C$ pretreatment was 2 days and longer time had deleterious effect on induction of microspore embryo.

고추의 약을 배지에 치상한 후 4$^{\circ}C$와 32$^{\circ}C$의 온도 전처리가 소포자의 활력, 초기분열 및 소포자 배 발생에 미치는 영향을 조사하였다. 활력 있는 소포자의 비율은 모 식물에서 채취하여 치상할 당시에는 62~64%정도였으나 치상 후 온도처리 기간 중에는 매우 급속하게 저하되었다. 소포자의 활력은 4$^{\circ}C$ 처리에 비해 32$^{\circ}C$ 처리 시에 더욱 심하게 감소하였으며 배양 9일이 되면 온도처리와 관계없이 활력 있는 소포자가 거의 없었다. 치상 당시의 소포자는 대부분이 후기 1핵성 소포자기였으며 배양 2일 후부터 핵이 소실된 무핵 소포자들과 함께 다양한 유형의 다핵 소포자들이 나타났다. 배 발생적 소포자의 비율은 고온처리에서 높았으나 균등분열에 의한 동형2핵 소포자의 출현에는 온도처리에 따른 차이가 크지 않았으며 다핵 소포자들은 균등 또는 불균등 분열에 의해 생겨났다. $25^{\circ}C$와 32$^{\circ}C$ 처리에서는 배양 2일 후에 이미 퇴화 소포자들이 50% 이상이 되었는데 4$^{\circ}C$ 처리에서는 14일 동안 대부분의 소포자들에서 핵이 완전하였다. 소포자 배의 발생은 4$^{\circ}C$ 처리에 비해 32$^{\circ}C$ 처리에서 높았으며 가장 효과적인 고온처리 기간은 4일이었다. 이에 비해 4$^{\circ}C$ 처리에서는 2일 처리가 가장 효과적이었으며 처리기간이 길어질수록 소포자 배의 발생이 감소되었다.

Keywords

References

  1. Plant Cell Tissue Org Cult v.20 The influence of various physical parameters in anther culture of Broccoli (Brassica pleracea var. italica) Arnison PG;Donaldson P;Ho LCC;Keller WA
  2. Plant Cell Tissue Org Cult v.25 Ethylene production during anther culture of brussels sprouts (Brasseica oleracea var. gemmigera) and its relationship with factors that affect embryo production Biddington NL;Robinson HT
  3. Plant Cell Tissue Org Cult v.41 Doubled haploid sunflower (Helianthus annuus) plant production by androgenesis: fact or artifact? Part 2. In vitro isolated microspore culture Coumans M;Shong D
  4. Acta Hort v.407 Regulation of inductive phase of microspore embryyogenesis in Brassica napus Custers JBM;Cordewener JHG;Dons HJM;Campagne MML
  5. Agronomie v.1 In vitro culture of pepper (capsicum annuum L.) anthers;high rate plant production from different genotypes by +35℃ treatment Dumas de Vaulx R;Chambonnet D;Pochard E
  6. Plant Cell Tissue Org Cult v.26 Heat shock response during anther culture of broccoli (Brassica oleracea var italica) Fabijanski SF;Altosaar I;Arnison G
  7. Protoplasma v.147 Development of microspore in vivo and vitro in Brassica nalus L. Fan Z;Armstrong C;Keller WA
  8. Plant Cell Rep v.10 Optimization of maize microspore isolation and culture conditions for reliable plant regeneration Gaillard A;Vergne P;Beckert M
  9. Plant Cell Tissue Org Cult v.42 Isolated wheat microspore culture Gustafson VD;Baenziger SP;Wright MS;Stroup WW;Yen Y
  10. J Plant Physiol v.156 Doubled haploid development and PCR-analysis of $F_1$ hybrid derived DH-R2 paprika (Capsicum annuum L.) lines Gyulai G;Gemesne A;Sagi Zs;Venczel G;Pinter P;Kristof Z;Torek O;Heszky L;Bottka S;Kiss J;Zatyko L
  11. Cell Biol Intl v.17 Cytoskeletal changes in induction of embryogenesis in microspore and pollen cultures of Brassica napus L. Hause B;Hause G;Pechan P;VanLammeren AAM
  12. Theor Appl Genet v.67 The evaluation of pollen quality and of further appraisal of the fluoro-chromatic (FRC) test procedure Heslop-Harrison J;Heslop-Harrison Y;Shivanna KR
  13. Plant Cell Tissue Org Cult v.49 Cytological analysis of early microspore divisions and embryo formation in dultured soybean anthers Kaltchuk-Santos E;Mariath JE;Mundstock E;Hu C;Bodanese-Zanettini MH
  14. Plant Cell Rep v.12 Viability, cell division and microcallus formation of barley microspores in culture Kao KN
  15. Can J Bot v.55 Embryogenesis and plant regeneration in Brassica napus anther cultures Keller WA;Armstrong KC
  16. Theor Appl Genet v.55 Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatment Keller WA;Armstrong KC
  17. Plant Cell Tissue Org Cult v.33 Anther culture of kale (Brassica oleracea L. convar. asephala (DC.) alef.) Kieffer M;Fuller MP;Chauvin JE;Schlesser A
  18. Kor. J Plant Tiss Cult v.26 The influence of temperature pretreatment on the production of microspore embryos in anther culture of Capsicum annuum L. Kim MZ
  19. J Plant Biotechnology v.2 Rapid assement of microspore development stage in pepper using DAPI and ferric chloride Kim MZ;Jang IC
  20. Plant Cell Tissue Org Cult v.54 The effect of cold and heat pretreatments on anther culture response of Avena sativa and A. sterilis Kiviharju E;Pehu E
  21. Plant Sci v.57 The influence of different temperature treatments on anther culture response of spring wheat (Triticum aestivum L.) Li H;Qureshi JH;Kartha KK
  22. Plant Physiol v.111 Complexity and genetic variability of heat-shock protein expression in isolated maize microspores Magnard JL;Vergene P;Dumas C
  23. Plant Cell Tissue Org Cult v.3 The influence of anther cold pretretments and donor plant genotypes on in vitro androgenesis in wheat Marsolais AA;Seguin-Swartz G;Kasha KJ
  24. Can J Plant Sci v.70 The influence of various cultural factors on anther culture of four cultivars of spring wheat (Triticum aestivum L.) McGregor LJ;McHughen A
  25. In Vitro Cell Dev Biol v.33 Development of asparagus microspores in vivo and in vitro is influenced by gametogenic stages and cold treatment Peng M;Ziauddin A;Wolyn DJ
  26. In vitro haploid production in higher plants v.3 Anther and microspore culture in capsicum Regner F;SM Jain(ed.);SK Sopory;RE Veilleux(ed.)
  27. Plant Cell Tissue Org Cult v.59 Manipulation of division symmetry and developmental fate in cultures of potato microspores Rihova L;Tupy J
  28. Sex Plant Reprod v.12 A new procedure to asses pollen viability Rodriguez-Riano T;Dafni A
  29. Physiol plant v.110 Stress, as assessed by the appearance of sHsp transcripts, is required but not sufficient to initiate androgenesis Smykal P;Pechan PM
  30. Protoplasma v.158 Initiation of non-physiological division and manipulation of developmental pathway in cultured microspores of Medicago sp Tanner GJ;Piccirilli M;Moore AE;Larkin PJ;Arcioni S
  31. Plant Cell Rep v.16 Microspore culture of radish (Raphanus sativus L.): influence of genotype and culture conditions on embryogenesis Takahata Y;Komatzu H;Kaizuma N
  32. Topas. Protoplasma v.185 Cellular changes during heat shock induction and embtyo development of cultured microspore of Brassica napus cv Telmer CA;Newcomb W;Simmonds DH
  33. Plant Cell Tissue Org Cult v.30 The influence of culture conditions on anther culture response of commercial varieties of Solanum tuberosum L Tiainen T
  34. Plant Cell Rep v.15 Stress-induced microspore embryogenesis in tobacco; an optimized system for molecular studies Touraev A;Ilham A;Vicente O;Heberle-Bors E