Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Gene Analysis of A Fruit-specific Thaumatin-like Protein, VVTL1-homolog, from Campbell Cultivar of Grape

포도 캠벨 품종으로부터 과육 특이발현 VVTL1-homolog 유전자의 분석

  • 김인중 (한국과학기술원 생물과학과) ;
  • 김석만 (한국과학기술원 생물과학과)
  • Published : 2001.09.01
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Abstract

Vitis vinifera thaumatin-like protein (VVTL1) is a fruit-specific and ripening-related protein in grape. In order to isolate VVTL1-homolog gene and fruit-specific promoter from Campbell cultivar, we isolated a genomic clone containing VVTL1-homolog gene from grape genomic library through plaque hybridization. VVTL1-homolog gene has an intronless genomic structure, which the pattern is matched with those of other PR5 genes such as osmotin and osmotin-like protein genes. Transcription start site was determined by primer extension analysis. The promoter region of VVTL1-homolog gene contains a sequence or structure, especially the location and number of TCA box and ABRE (abscisic acid-responsive element), distinct from other reported plant PR5 genes, though with several known functional elements such as a TATA box and CAAT box. These results suggested that VVTL1-homolog gene may be regulated by a plant hormone, abscisic acid, and one or several stresses such osmotic pressure and pathogen infection. The isolation of fruit-specific promoter may be helpful to breed a genetically modified grape with valuable phenotype or materials in fruits.

VVTL1은 포도 과육에서 특이적으로 다량 발현되는, PR5 계열의 thaumatin과 높은 상동성을 나타내는 단백질로서, 품종에 따라 염기서열의 차이를 나타낸다고 알려져 있다. 현재까지 포도의 VVTL1에 대해서 몇몇 연구가 진행되었지만, 우리나라에서 가장 많이 재배되는 품종인 캠벨에서는 전혀 이루어지지 않았다. 본 연구에서는 캠벨 품종으로부터 VVTL1-homolog 유전자의 게놈 DNA를 분리하여, 염기서열을 분석하였다. VVTL1-homolog 유전자는 일반적으로 PR5 유전자의 구조와 동일한 구조인, intron이 없는 하나의 exon으로만 구성되어 있었다. 염기서열로부터 추론된 VVTL1-homolog 단백질의 아미노산 서열은 VVTL1을 비롯한 다른 품종의 포도에서 분리된 TLP와는 달리 염기성의 등전점을 가지고 있었다. Primer extension 분석을 통해 전사개시 부위를 결정하였고, promoter영역을 포함하는 5'upstream 지역에 전사에 중요한 TATA box (4개)와 CAAT box (1개)가 존재하였으나, 이들의 위치와 수는 다른 PR5 유전자의 promoter와는 다랐다. 이러한 연구결과는 VVTL1-homolog 유전자의 발현이 과육 성숙과정동안 abscisic acid와 스트레스 또는 자극에 의해 발현이 유도되고 있음을 제시해준다. 포도 과육특이발현 promoter인 VVTL1-homolog 유전자의 promoter 분리는, 유전자의 도입에 의해 유용형질을 과육에 나타내는 포도품종을 개발하고자 할 때 효율적으로 사용될 수 있을 것으로 사료된다.

Keywords

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