Effects of Cross-Pretreatment of Cadmium and Arsenic on Lethality of Cadmium or Arsenic to Mice

카드뮴과 비소의 생쥐 치사독성에 대한 카드뮴과 비소의 교차전처리효과

  • 부문종 (삼육대학교 생명과학과)
  • Published : 2001.06.01

Abstract

Whether the pretreatment of sublethal arsenic or cadmium may prevent from lethality of arsenic or cadmium to mice, respectively, and also the protection against to lethality of arsenic or cadmium which might be induced by pretreatment of arsenic or cadmium may be related with their hepatic glutathione contents were investigated. When sodium arsenite or cadmium chloride was subcutaneously injected to mice (ICR strain) using lethal doses, all mice of both group were killed. The mortality of mice which were subsequently injected with lethal arsenic 24 hours after pretreatment of sublethal arsenic was decreased, and the same result was obtained in the case of cadmium. Sublethal pretreatment of arsenic or cadmium prior to lethal arsenic or cadmium treatment to mice, respectively, didn't decrease hepatic glutathione contents of the survived mice, while decreases of that contents in liver were observed in the mice just after they died. Cadmium pretreatment decreased mortality of mice which subsequently injected with lethal arsenic, while arsenic pretreatment didn't protect against cadmium lethality. These results indicate that protection against arsenic or cadmium lethality to mice induced by pretreatment of sublethal arsenic or cadmium may be directly related to other factors induced by sublethal camium pretreatment, not to hepatic glutathione contents.

생쥐에서 카드뮴이나 비소의 전처리에 의하여 카드뮴과 비소의 치사독성이 완화되는 효과가 카드뮴과 비소를 교차 전처리 한 경우에도 이 같은 효과가 나타나는지를 조사하였다 치사량의 비소를 주사하기 전에 치사량 이하의 비소를 생쥐에 주사하면 치사량의 비소에 의한 치사작용이 완화되는 효과가 나타나며 카드뮴 전처리에 의해서도 카드뮴의 치사독성은 완화되었다. 두 경우에서 생존한 개체의 간조직의 glutathione함량은 대조군과 별 차이가 없었으나 치사한 개체는 그 함량이 상당량 감소하였다. 이러한 결과는 카드뮴이나 비소의 전처리에 의하여 카드뮴과 비소의 치사독성이 완화되는 효과가 간조직의 glutathione 함량과 관련이 있음을 제시한다. 치사량의 카드뮴을 주사하기 24시간 전에 치사량 이하의 비소를 전처리한 경우에 카드뮴의 치사독성은 완화되지 않았으나 치사량의 비소를 주사하기 24시간 전에 카드뮴을 전처리한 경우에 비소의 치사독성은 완화되었다. 이상과 같은 실험결과는 간조직의 glutathione 함량이 카드뮴이나 비소의 치사독성 완화효과에 직접적인 관련성은 있는 것이 아니라, 카드뮴 전처리에 의하여 유도된 어떤 다른 인자들이 후속 주사하는 치사량의 카드뮴에 의한 치사독성을 완화하고, 이 인자들 중에는 비소의 치사독성도 완화할 수 있는 공통의 인자가 존재함을 암시한다.

Keywords

References

  1. Arch. Biochem. Biophys. v.382 Cadmium-induced mRNA expression of Hsp32 is augmented in metallotnionein-Ⅰand -Ⅱ knock-out mice Abe T;O Yamamoto;S Gotoh;Y Yan;N Todaka
  2. Free Radic. Boil. Med. v.29 Exposure of development oligodendrocyte to cadmium causes HSP72 induction, free radical generation, reduction in glutathione levels, and cell death Almazan G;H Liu;A Khorchid;S Sundarajan;Ak Martinez-Bermudez;S Chemtob
  3. Chem. Biol. Interact. v.85 Arsentic induces and enhances rat hepatic metallothionein production in vivo Alvores A;J Koropatnick;MG Cherian;AJ Zelazowaki
  4. J. Inorg. Biochem. v.54 Interaction of selenium and arsenic with metallothionein: effect of vitamin B12 Chen CL;PD Whanger
  5. Toxicology v.150 Tolerance to cadmium hepatotocixity by metallothionein and zinc: in vivo and in vitro studies with MT-null mice Coyle P;G Niezing;TL Shelton;JC Philcox;AM Rofe
  6. Toxicology v.116 Sequence of exposure to cadmium and arsenic determines the extent of toxic effects in male Fischer rats Hochadel JF;MP Waalkes
  7. J. cell. Physiol. v.115 Induction of thermotolerance and enhanced heat shock protein synthesis in chinese hamster fibroblasts by sodium arsenite and by ethanol Li GC
  8. Fundam. Appl. Toxicol. v.27 Reversal of oxophenylarsine-induced inhibition of glucose uptake in MDCK cells Liebl B;H Muckter;E Doklwa;B FIchtl;W Forth
  9. Arch. Toxicol. v.69 Influence of glucose on the toxicity of oxophenylarsine in MDCK cells Liebl B;H Muckter;E Doklea;FX Reichl;B Fichtl;W Forth
  10. Toxicol. Sci. v.57 Metallothionein-Ⅰ/Ⅱ null mice are sensitive to chronic oral cadmium-induced nephrotoxicity Lui Y;J Lui;SM Habeebu;MP Waalkes;CD Klassen
  11. Biochim. Biophys. Acta v.628 Induction of mucosal glutathione synthesis by arsenic Picioto PT;JH Graziano
  12. Vet. Hum. Toxicol. v.33 Effects of glucose treatment on carbohydrate content in various organs on mice after acute As₂O₃ poisoning Reichl FX;H Kreppel;L Szinicz;B Fichtl;W Forth
  13. Arch. Toxicol. v.64 Effect of glucose in mice after acute experimental poisoning with arsenic trioxide (As₂O₃) Reichl FX;L Szinicz;H Kreppel;B FIchtl;W Forth
  14. Toxicol. Sci. v.54 Studies on the mechanisms of arsenic-induced self tolerance developed in liver eqithelial cells through continuous low-level arsenite exposure Romach EH:CQ Zhao;LM Del;ME Chebrian
  15. Toxicol. Appl. Pharmacol. v.141 Induction of hsp70 in HepG2 cells in response to hepatotoxicants Salminen WFJr;R Voellmy;SM Roberts
  16. Methods in Enzymology v.113 Gllutathione biosynthesis Selig GF;A Meister;Meister A(ed.)
  17. Toxicol. Sci. v.45 Effect of glutathione depletion and metallothionein gene expression on arsenic-induced cytotoxicity adn c-myc expression in vitro Shimizu M;JF Hochadel;BA Fulmer;MP Waalkes
  18. Toxicology v.151 Cadmium-induced elevations in the gene expression of the regulatory subunit of gamma-glutamylcysteine synthetase in rat lung and alveolar epithelial cells Shukla GS;J Chiu;BA Hart
  19. FASEB J. v.1 Glutathione, a first line defense against cadmium Singhal RK;ME Anderson;A Meister
  20. Jpn. J. Med. Sci. Biol. v.49 Realtionship between glutatione and DL alpha-lipoic acid against cadmium-induced hepatotoxicity Sumathi R;G Baskaran;P Varalakshmi
  21. Arch. Toxicol. v.61 Effect of As₂O₃ on gluconeogenesis Szinicz L;W Forth
  22. FEBS Lett. v.245 The human 32-lDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase Taketani S;H Kohno;T Yoshinaga;R Tokunaga