Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Vitellogenin mRNA Induction in Male Carp Treated with Bisphenol A

비스페놀A 처리 수컷 잉어에서 비텔로제닌 mRNA의 발현

  • 박응로 (국립환경연구원 환경위해성연구과) ;
  • 최필선 (국립환경연구원 환경위해성연구과) ;
  • 이철우 (국립환경연구원 환경위해성연구과) ;
  • 류지성 (국립환경연구원 환경위해성연구과) ;
  • 남성숙 (국립환경연구원 환경위해성연구과) ;
  • 류홍일 (국립환경연구원 환경위해성연구과) ;
  • 전성환 (국립환경연구원 환경위해성연구과) ;
  • 나진균 (국립환경연구원 환경위해성연구과) ;
  • 박광식 (국립환경연구원 환경위해성연구과)
  • Published : 2001.10.01
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Abstract

The estrogenic potency of bisphenol A using reverse trancriptase-PCR response of liver vitellogenin mRNA in male carp was studied. For this, six combination of primers which were synthesized on the basis of cDNA consensus region of various species, were evaluated and one pair of primers was selected as the best to show 286 bp size-transcript. By using the selected primers, vitellogenin mRNA induction in carp treated with bisphenol A was measured and the chemical showed dose-and time-dependent Induction response. From this result, it was concluded that RT-PCR technique wing the selected primers in this study can be wed to monitor the estrogenic effects exerted In carp living in Korean freshwater.

Keywords

References

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