The Protective Effects of the Extract of Saururus chinesis against Cadmium Induced Cytotoxicity(II)

삼백초 추출물의 카드뮴독성에 대한 방어효과(II)

  • 이정호 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 유일수 (익산대학 공업화학과) ;
  • 이기남 (원광대학교 산업한의학교실) ;
  • 지정목 (원광대학교 산업한의학교실) ;
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 신민교 (원광대학교 한의과대학 본초학교실) ;
  • 정승일 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 오현주 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2001.10.01

Abstract

This study was conducted to investigate the antitoxic component in ethanol extract of Saururus chinesis (S. chinesis). The results were as follows: Generally, detoxication effects by s. chinesis extract increased in proportion to the extract concentration. non 8 $\mu\textrm{g}$/g dosage of S. chinesis extract was administered, it showed the highest antitoxic effects in metallothionein induction. After the extract treatment, body weights generally increased In proportion to the extract concentrations. from the above results, S. chinesis extract Increased Metallothionein concentration and decreased the toxicity of cadmium In rats. In vitro the antitoxic activity of ethanol extract of S. chinesis on NIH3T3 fibroblasts was evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) and SRB (sulforhodamine B protein) assays. The light microscopic study was carried out to observe morphological changes of the treeated cells. $10^{-2}$mg/ml Concentrations of S. chinesis extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were increased and tend to regenerate. These result suggest that S. chinesis extract retains a potential antitoxic activity.

Keywords

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