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The Pharmaceutical Society of Korea (대한약학회)
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Determination of Recombinant Human Epidermal Growth factor (rhEGF) in a Pharmaceutical Preparation by Capillary Electrophoresis

  • Published : 2001.12.01
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Abstract

A simple assay method of recombinant human epidermal growth factor (rhEGF) in a pharmaceutical preparation was studied and validated by capillary electrophoresis (CE) using micellar electrokinetic chromatography (MEKC) techniques. Factors affecting the migration behavior and separation performances of the peptide; type of buffers pH, butler concentration, and concentration of sodium dodecyl sulfates (SDS) were investigated to optimize the analytical performance. CE was performed using running buffers 50.0 mM borate (pH 8.5) containing 12.5 mM SDS at 20 $mutextrm{V}$ of the applied voltage. Calibration curves for the rhEGF showed good linearity (r>0.999) over the wide dynamic range from 1.25 to $100{\mu\textrm{g}}/ml$. Sample analysis was performed by using standard addition method to eliminate the matrix effects of dosage vehicle. This method is assumed to be useful for quality control (QC) of various forms of pharmaceutical products of the peptide.

Keywords

References

  1. Surg. v.18 Epidermal growth factor in the healing of corneal wound Arturson,G.
  2. J. Exp. Med. v.163 Enhancement of epidermal regeneration by biosynthetic EGF. Chiron. Wound healing efficacy Brown,G.L.;Curtsinger,L.;Brightwell,J.R.;Ackemen,D.M.;Tobin,G.R.;Polk,H.C.;George-Nascimento,C.;Valenzuela,P.;Schultz,G.S.
  3. New Eng. J. Med. v.321 Enhancement of wound healing by topical treatment with epidermal growth factor Brown,G.L.;Nanney,L.B.;Griffen,J.;Cramer,A.B.;Yancey,J.M.;Curtsinger,L.J.;Holtzin,L.;Schultz,G.S.;Jurkiewicz,M.J.;Lynch,J.B.
  4. Annu. Rev. Biochem. v.48 Epidermal growth factor Carpenter,G.;Cohen,S.
  5. J. Burn Care Rehabil v.9 Lanolin and epidermal growth factor in healing of partial thickness pig wounds Chvapil,M.;Gaines,J.A.;Gilman,T.
  6. J. Biol. Chem. v.237 Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal Cohen,S.
  7. Pharm. Res. v.2 Peptides and proteins as drugs Ferraiolo,B.L.;Benet,L.Z.
  8. J. Lab. Clin. Med. v.108 Accerleration of wound healing by recominant human urogastrone(epidermal growth factor) Gregory,T.J.F.H.;Morris,W.P.
  9. J. Clin. Endocrinol. Metab. v.48 Epidermal growth factor (urogastrone) in human tissues Hirata,Y.;Orth,D.N.
  10. J. Clin. Endocrinol. Metab. v.48 Epidermal growth factor (urogastrone) in human fluids :size heterogeneity Hirata,Y.;Orth,D.N.
  11. Gastroenterology v.81 Gastric cytoprotection by epidermal growth factor. Konturek,S.J.;Radecki,T.;Brzozowski,T.
  12. Gut. v.22 Role of mucosal prostaglandins and DNA synthesisin gastric cytoprotection by luminal epdermal growth factor Konturek,S.J.;Brzozowski,T.;Piastucki,I.
  13. Anal. Chem. v.58 Capillary zone electrophoresis of proteins in untreated fused silica tubings Lauer,H.H;McManigill,D.
  14. Ann. Rev. Physiol. v.48 Effect of growth factors on intrcellular pH regulation Moolenaar,W.M.
  15. Pharm. Res. v.7 Improvement in wound healing by epidermal growth factors(EGF) Ointment Okumra,K.;Kiyohara,Y.;Komada,F.;Iwakawa,S.;Hirai,M.;Fuwa,T.
  16. Am. J. Physiol. v.22 Processing of epidermal growth factors by suckling and adult rat intestinal cells Rao,R.K.;Thornburg,W.;Korc,M.;Matricisian,L.M.;Magun B.E.;Koldovsky,O.
  17. Science v.235 Epithelial wound healing enhanced by transforming growth factor-alpha and vaccinia growth factor Schultz,G.S.;White,M.;Mitchell,R.;Brown,G.;Lynch,J.;Twardzik,D.R.;Todaro,G.J.
  18. J. Cellular Biochem. v.45 EGF and TGF-alpha in wound healing and repair Schultz,G.;Hotatori,D.S.;Clatk,W.
  19. Am. J. Ophthal. v.103 Epidermal growth factor in alkali-burned corneal epithelial wound healing Singh,G.;Foster,C.
  20. Science v.189 Epidermal growth factor: Identification of a new hormone in human urine Starkey,R.;Cohen.S.;Orth,D.N.
  21. Cancer Res. v.46 Role of epidermal growth factor in carcinogenesis Stoscheck,C.M.;Knig,I.E.
  22. Mol. Cell. Endocrinol. v.47 Shrew subaxillary gland epidermal growth factor: homologous radioreceptor assay and mitogenic activity Yip,T.T.;Tsao,S.W.;Ko,K.M.;Xin,J.X.;Kong,Y.C.