Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
권호 표지

Studies on the Cold Attenuation and Protective Effects of a Thermostable Newscastle Disease Virus Isolated from Korean Pheasants

한국산 꿩으로부터 분리한 열안정성 뉴캣슬병 바이러스의 저온순화와 방어효과

  • K. H. Kwak (College of Veterinary Medicine, Chonbuk National University) ;
  • S. C. Han (College of Veterinary Medicine, Chonbuk National University) ;
  • T. J. Kim (Bio-Safety Research Institute, Chonbuk National University) ;
  • K. S. Chang (College of Veterinary Medicine, Chungnam National University) ;
  • M. H. Jun (College of Veterinary Medicine, Chungnam National University) ;
  • H. J. Song (Bio-Safety Research Institute, Chonbuk National University)
  • Published : 2001.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Newcastle disease virus, CBP-1 strain isolated from Korean pheasants was passaged for 173 times by 9-day-old specific pathogenic free (SPF) embryonated eggs at $37^{\circ}C$ (parent strain) and subsequently passaged for 15 (cold attenuation (CA) -15) and 30(cold attenuation (CA) -30) times by 10-day-old of commercial broiler chicks embryonated eggs at $29^{\circ}C$, respectively, The Physical and chemical properties (sensitivity to lipid solvents, low pH and thermostability), pathogenicity (mean death time, intracerebral pathogenic index and intravenous patho-genic index), safety, booster or protective effect and characterization of temperature sensitivity were measured in cold attenuated CA-15 or 30 strain and compared to those of parent CBP-1 strain. NDV, CBP-1 CA-30 strain acquired cold attenuation and decreased infectivity at $41^{\circ}C$ compared to those of parent strain grown at $37^{\circ}C$. It lost hemagglutination activity (HA) and cell infectivity at $56^{\circ}C$ for 30, 60, and 120 Min. CA-30 strain treated with ethyl ether also lost its HA and cell infectivity. Both CA-30 and parent strains exhibited a little resistant to HA at pH 3.0 glycine HCI buffer. Intracerebral pathogenic index (ICPI) and intravenous pathogenic index (IVPI) of parent strain were 1.12 and 1.45, but decreased to 0.75 and 0.00 in CA-30 strain, respectively. The safety was evaluated by mortality in chicks inoculated with 10$^{4.0}$ EID$_{50}$ /0.1 ml. The mortalities of parent, CA-30 and commercial Bl strains were 17.5, 12.0 and 0.0%, respectively. The safety of CA-30 strain was higher than that of parent strain. The booster effects of CA-30 strain and parent strain performed in 4-week-old chicks after being vaccinated with primary commercial Bl strain.

열 안정성을 가지고 있는 한국산 꿩에서 분리된 New-castle disease virus CBP-1주는 9일령 SPF 계태아에 접종되어 $37^{\circ}C$에서 배양하는 방법으로 173번(parent주) 누대 배양되었다. $37^{\circ}C$에서 173번 누대 배양된 NDV CBP-1 주를 10 일령 계태아에 접종한 후 저온에서 ($29^{\circ}C$) 15번 (CA-15) 30번(CA-30) 누대 배양하였다. 저온순화 주인 CA-15주와 CA-30주의 이화학적 성상검사 (열 안정성 실험, 지질 용매에 대한 감수성 실험, 산성 용매에 대한 감수성 실험)와 병원성 실험(MDT, ICPI, IVPI), 온도 감수성 실험, 안전성 실험, 부스터 효과 실험, 방어효과 등을 실험하였고, $37^{\circ}C$에서 173번 누대 배양된 parent주와 비교하였다. $29^{\circ}C$에 적응된 CA-30주는 $37^{\circ}C$와 $41^{\circ}C$에서 세포 감염력이 parent주와 비교할 때 감소하였다. CA-15주와 CA-30주를 $56^{\circ}C$에서 30분, 60분, 120분 동안 처리하였을 때 이들 저온 순화주들은 혈구응집능과 세포감염력을 상실하였다. Parent주와 CA-15, CA-30주는 ethyl ether를 10분간 처리했을 때 혈구응집능과 세포 감염력을 모두 상실하였다. 그러나 parent주와 CA-15, CA-30주는 pH 3.0-glycine HCl 완충액에 60분간 처리하였을 때 혈구응집능을 가지고 있었다. Parent주의 대뇌병원성 지수와 정맥내 병원성 지수는 각각 1.12, 1.45 이었다. 그러나 CA-30주의 대뇌 병원성 지수와 정맥내 병원성 지수는 각각 0.75, 0.00으로 감소하였다. CA-30주의 안전성은 1 일령 병아리에서 실시하였고 parent주와 B-1주와 비교였다. 이들의 안전성은 치사율을 가지고 평가하였다. parent주와 CA-30주와 B-1주의 치사율은 각각 17.5, 12.0. 0.0%이었다. CA-30주가 parent주에 비해 보다 높은 안전성을 보여 주었으나 B-1주에 비해서 아직 높은 치사율을 보여 주고 있었다.

Keywords

References

  1. Acute virus infections of poultry The classfication, host range and distribution of avian paramyxovirus Alexander,D.J.;Mferran,J.B.(ed.);McNulty,M.S.(ed.)
  2. Newcastle disease Newcatle disease methods of spread Alexander,D.J.;Alexander,D.J.(ed.)
  3. Manual of standards for diagnostic tests and vaccines(3rd ed.) Alexander,D.J.
  4. Disease of poultry(10th ed.) Alexander,D.J.
  5. Avian Pathol v.3 Newcastle Disease virus pathotypes Alexander,D.J.;Allan,W.H.
  6. Disease of poutry(8th ed.) Newcastel disease Beard,C.W.;Hanson,R.P.;Hosfstad,M.S.(ed.);Barnes,H.J.(ed.);Calnek,B.W.(ed.)
  7. Virology a laboratory manual Burleson,F.G.;Chambers,T.M.;Wiedbrauk,D.L.
  8. Avian Dis v.40 Attenuation of lentogenic Newcastle disease virus strain B1 by cold adaptation Gelb,J.Jr;Lunt,R.L.;Metz,A.L.(et al.)
  9. Avian Dis v.35 Attenuation of avian infectious brochitis viurs by cold-adaption Gelb,J.Jr;Lunt,R.L.;Ramos,T.(et al.)
  10. Virology v.126 In vitro characterization of an avian reovirus vaccine strain Govea,V.;Huang,D.D.;Ramos,T.(et al.)
  11. Science v.122 Identification of vaccine strains of Newcastle disease virus Hanson,R.P.;Brandly,C.A.
  12. Proc Soc Exp Biol Med v.70 Heat stability of hemagglutinin of various strains of Newcastle disease virus Hanson,R.P.;Upton,E.;Brandly,C.A.(et al.)
  13. Korean J Poult Sci v.27 Attenuation and protective effects of a thermostable Newcastle disease virus isolated from Korean pheasants Han,S.C.;Kwak,K.H.;Kim,T.J.;Chang,K.S.;Jun,M.H.;Song,H.J.
  14. Ned Indisch BI Diergeneeskd v.38 A poultry disease in the Dutch East Indies Kraneveld,F.C.
  15. Monograph no.3 Newcastle disease. a review1926-1964. Lancaster,J.E.
  16. Nature v.213 Adaptation and growth of influenza virus at 25℃ Maassab,H.F.
  17. Kor J Vet Publ Hlth v.19 Studies on the incidence and isolation of Newcastle disease virus in pheasant flocks Park,J.W.;Chang,K.S.;Jun,M.H.