한국수의병리학회지 (Korean Journal of Veterinary Pathology)

한국수의병리학회 (Korean Society of Veterinary Pathology)
권호 표지

In situ Hybridization에 의한 토끼출혈증(rabbit haemorrhagic disease)의 신속.간편한 진단

Rapid and Easy Diagnosis of Rabbit Haemorrhagic Disease by In Situ Hybridization

  • 박남용 (전남대학교 수의과대학 수의병리학교실) ;
  • 조호성 (전남대학교 수의과대학 수의병리학교실) ;
  • 조경오 (전남대학교 수의과대학 수의병리학교실) ;
  • 김상집 (전남대학교 수의과대학 수의병리학교실) ;
  • 박형선 (전남대학교 수의과대학 수의병리학교실)
  • Park, Nam-Yong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Cho, Ho-Seong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Cho, Kyoung-Oh (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sang-Jip (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Park, Hyung-Seon (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University)
  • 발행 : 2001.12.01
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초록

Recently various molecular diagnostic techniques have been used to identify rabbit hemorrhagic disease virus (RHDV), a causative agent responsible for acute hepatitis and disseminated intravascular coagulation in rabbit. But they were hard to perform and time consuming. To detect RHDV in a rapid and easy way, we developed biotinylated oligonucleotide probe within ORF 1 region encoding the polyprotein of RHDV in formalin-fixed and paraffin-embedded tissues from various tissues of 20 rabbits naturally infected with RHDV, Our in situ hybridization (ISH) was quickly carried out within two hours by MicroProbe capillary action system. The ISH produced a positive reaction in liver, kidney and lung. In conclusion, ISH with a biotintlated oligonucleotide probe provided a useful diagnostic method for detecting RHDV.

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