Pseudomonas cepacia KH410의 중금속 흡착특성

Characteristics of Heavy Metal Biosorption by Pseudomonas cepacia KH410

  • 박지원 (동의대학교 미생물학과) ;
  • 김영희 (동의대학교 미생물학과)
  • 발행 : 2001.09.01

초록

담수식물의 근계(根界) 부착하는 미생물 군집에서 중금속 제거능이 있는 균주인 Pseudomonas. cepacia KH410 을 분리하여 이 균주의 납과 카드윰, 구리에 대한 생흡착 특성을 조사하였다. 최적 흠착조건은 1.0 g-biomass, pH 4, 그리고 온도는 $40^{\circ}C$일 때이었다. 흡착평형은 120분에서, 1000 mg/1농도에서 이루어 졌다. 흡착용량(K)은 납이 카드뮴에 비해 5.6배, 구리에 비해서는 4.0배 높았으며 흡착강도(1/n)는 납>구리>카드뮴의 순이었다. 흠작강도에 따른 등온식 적용은 납은 Langmuir등온식, 그리고 구리와 카드뮴은 Freundlich 등온식 적용이 오차가 적었다. 건조 균체를 이용한 최대 흡착은 납과 카드뮴, 구리에 대하여 각각 83.2, 42.0, 65.2 mg/g-biomass 이었다. 중금속 회수를 위한 탈착 실험에서는 납은 0.1 M HCl에서 그리고 카드뮴과 구리는 0.1 M $HNO_3$에 의하여 높은 탈착율을 나타내었다. 고정화 균체의 강도를 높이기 위한 전처리는 각각의 세 가지 중금속에 대하여 0.1 M KOH가 가장 효율적이었다. 고정화 균체에 의한 최대 흡착은 납, 카드뮴, 구리가 각각 77.8, 58.5, 71.2 mg/g-biomass 이었으며 경금속 혼재시에도 비교적 안정한 상태를 나타내었으며 중금속 제거효율을 비교한 결과 고정화 균체가 이온교환수지에 의한 제거보다 높았다.

An ubiquitous bacterium, Pseudomonar cepacia KH410 was isolated from fresh water plant root and identified. Adsorption of heavy metals of lead, cadmium and copper by this strain was investigated. Optimal conditions foradsorption was 1.0 dry g-biomass, at pH 4.0 and temperature of $40^{\circ}C$. Adsorption equilibrium reached max-imum after 120 min in 1000 mg/l metal solutions. The adsorption capacity (K) of lead was 5.6 times higher thancadmium and 4.0 times higher than that of copper. Adsorption of lead was applicable for Langmuir modelwhereas Freundlich model for cadmium and copper, respectively. Adsorption strength (1/n) of heavy metal ionswere in the order of lead>copper>cadmium. Uptake capacity of lead, cadmium and copper by dried cell was83.2,42.0,65.2 mg/g-biomass, respectively. Effective desorption was induced 0.1 M HCI for lead and 0.1 $HNO_3$ for cadmium and copper. Pretreatment to increase ion strength was the most effective with 0.1 M KOH.Uptake by immobilized cell was 77.8,58.5,71.2 mg/g-biomass for lead, cadmium and copper, respectively. Theimmobilized cell was more effective than ion exchange resin on removal of heavy metals in solution containinglight metals.

키워드

참고문헌

  1. 생명과학회지 v.9 담수식물 뿌리로부터 세균의 분리 김영희
  2. J. Koren Environmental Sci. Soci. v.7 Removal of lead by Artherobacter sp. Ahn. K.H.;K.H. Suh
  3. J. Koren Environmental Sci. Soci. v.6 Removal, recovery, and process development of heavy metal by immobilized biomass methods Ahn, K.H.;Y.K. Shi;K.H. Suh
  4. Wastewater Microbiology Bitton, G.
  5. Appl. Microbiol. Biotechnol v.41 Bioaccumulation of metal cations by Saccharomyces cerevisiae Bready, D.;J.R. Duncan
  6. Biochemical Society Transactions v.26 Heavy metal resistance genes and proteins in bacteria and their application Brown, N.L.;J.R. Lloyd;K. Jakeman;J.L. Hobman;I. Bontidean;B. Mattiasson;E. Csoregit
  7. Current Microbiology v.41 Removal of $Pb^{2+}$ by biomass of marine algae Hamdy, A.A.
  8. Bioresource Technology v.65 Effects of heavy metals on Pseudomonas aeruginosa and Bacillus thuringiensis Hassen, A.;N. Saidi;M. Cherif;A. Boudabous
  9. J. Korean Environmental Sci. Soci. v.9 A variation of microorganism by the biosorption of $Pb^{2+}$ Kim, D.S.;J.H. Suh;S.K. Song
  10. Appl. Environ. Microbiol. v.65 Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides Kotrba, P.;L. Doleckova;V.D. Lorenzo;T. Ruml
  11. Appl. Environ. Microbiol. v.60 Uptake of zinc in Pseudomonas sp. Strain UDG26 Mago, R.;S. Srivastava
  12. Appl. Environ. Microbiol. v.55 Bacterial sorption of heavy metals Mullen, M.D.;D.C. Wolf;F.G. Ferris;T.J. Beveridge;C.A. Flemming;G.W. Bailey
  13. Appl. Environ. Microbiol. v.51 Microbial heavy-metal resistance Nies, S.H.
  14. Canadian J. analytical Sciences and Spectroscopy v.42 Biotransformation of cadmium species by microorganism Panichev, N.A.;A.O. Diakov;K.V. Kvitko
  15. Process Biochemistry v.31 Fully competitive biosorption of chromium and iron ions from binary metal mixtures by R.arrhizus: Use of the competitive Langmuir model Sag. Y.;T. Kutsal
  16. Bioprocess Engineering v.20 Effect of pH on $Pb^{2+}$ accumulation in Saccharomyces cerevisiae and Aureobasidium pullulans Suh, J.H.;J.W. Yun;D.S. Kim