Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation and Characterization of Bacteria Capable of Degrading Bisphenol A

Bisphenol A 분해세균의 분리 및 특성

  • 김희식 (한국생명공학연구원 생물공정연구실) ;
  • 이영기 (한국생명공학연구원 생물공정연구실) ;
  • 이완석 (한국생명공학연구원 생물공정연구실) ;
  • 박찬선 (한국생명공학연구원 생물공정연구실) ;
  • 윤병대 (한국생명공학연구원 생물공정연구실) ;
  • 오희목 (한국생명공학연구원 생물공정연구실)
  • Published : 2001.09.01
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Abstract

Eighty-seven microbial strains capable of growing on bisphenol A (BPA) as a sole carbon source were isolated from soils, waste waters and sludges. Among them, three bacterial strains were finally selected as potential decomposers through measuring BPA-degradation efficiency by HPLC analysis. Two of these bacterial strains were identified as Serratia marcescens 1901 and S. marcescens 1902, and another was Pseudomonas putida 1401 by 16S rDNA partial sequences and based on morphological and physiological properties. They showed higher cell growth and BPA degradation in PAV (PAS medium containing vitamin mixtures) than in PAS medium. The degradation efficiencies of these bacterial strains were within a range of 20-40% in the PAV containing 500 mg/1 or 100 mg/l of BPA fur 3 days. S. marcescens 1901 showed higher degradation efficiency at 100 mg/1 of BPA than those of other selected strains, while S. marcescens 1902 and P. putida 1401 degraded a high concentration of BPA (500 mg/l) with a degradation efficiency of 40% for 3 days. The BPA degradation using a mixed culture of three selected strains showed the similar level of dog-radation efficiency with that using a pure culture.

내분비계 장애물질로 잘 알려진 bisphenol A (BPA)를 단일탄소원으로 이용하여 균체성장을 나타내는 미생물 87주를 공단주변의 토양, 폐수 혹은 활성슬러지로부터 분리하였다. 분리된 균주 중 균체성장이 우수한 균주 8중을 2차 분리하였으며, 이 중 BPA분해효율이 뛰어난 3종의 균주를 BPA분해미생물로 최종 선별하였다. 최종 선별된 3종의 균주를 16s rDNA의 부분적 염기서열 및 형태학적, 생리학적 특성조사를 통해 Serratia maycescens 1901, S, marcescens 1902 그리고 Pseudomanas putida 1401로 동정되었다. BPA분해능은 HPLC분석을 통해 배양액중의 잔존 BPA 농도로 측정하였으며, 최종 선별된 3종의 균주를 대상으로 BPA가 100 mg/1흑은 500 mg/l의 농도로 포함된 최소 무기염 배지(PAS) 및 비타민을 포함하는 PAS인 PAV 배지에서 배양하여 BPA 분해능을 조사한 결과 20-40%의 분해효율을 나타내었다. 이들 균주의 균체성장은 PAS 배지에서 보다 PAV배지에서 우수하였다. S. marcescens 1901은 저농도(100 mg/l) BPA에서 분해효율이 다른 2종의 균주보다 우수하였고, S. marcescens 1902와 P. putida 1401은 고농도(500 mg/l)에서 BPA분해효율이 높았다. 선별된 3균주의 순수배양과 흔합배양에 의한 BPA분해효율을 비교한 결과, 유의한 차이는 나타나지 않았다.

Keywords

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