Abstract
The conversion of cephalosporin C to 7$\alpha$-hydroxycephalosporin C was examined with the cell-free extract of several cephamycin producing strains. Streptomyces clavuligerus ATCC 27064 was the most potent strain for the activity of cephalosporin 7$\alpha$-hydroxylase. Partially purified and immobilized cephalosporin 7$\alpha$-hydroxylase with resins were used to synthesize 7$\alpha$-hydroxycephalosporin C from the substrate, cephalosporin C. The molecular weight of the product isolated from the reaction mixture were determined to be 431 by ESI-Mass. $^1H$ NMR also support the conversion of cephalosporin C to 7$\alpha$-hydroxycephalosporin C by immobilized enzyme.
Cephamycin C를 생산하는 몇가지 균주를 사용하여 cephalosporin C로부터 7$\alpha$-hydroxycephalosporin C로의 전환력을 조사하였다. 이들 균주중에서 cephalosporin 7$\alpha$-hydroxylase의 활성은 Streptomyces clavuligerus ATCC 27064가 가장 높게 나타났다. Streptomyces clavuligerus ATCC 27064로부터 얻은 조효소액을 정제한 후 activated DEAE-sphacel 레진에 고정화하여 기질인 cephalosporin C로부터 7$\alpha$-hydroxycephalosporin C를 생산하였다. 고정화에 의해서 생성된 물질을 ESI-Mass로 측정한 결과, 분자량 431로 나타났다. 또한 $^1H$ NMR 분석에 의해 고정화 효소에 의해 생성된 물질은 cephalosporin C로부터 얻어진 7$\alpha$-hydroxycephalosporin C임을 확인하였다.