• Korean Journal of Microbiology
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• v.37 no.2
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• pp.164-169
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• 2001

The conversion of cephalosporin C to 7$\alpha$-hydroxycephalosporin C was examined with the cell-free extract of several cephamycin producing strains. Streptomyces clavuligerus ATCC 27064 was the most potent strain for the activity of cephalosporin 7$\alpha$-hydroxylase. Partially purified and immobilized cephalosporin 7$\alpha$-hydroxylase with resins were used to synthesize 7$\alpha$-hydroxycephalosporin C from the substrate, cephalosporin C. The molecular weight of the product isolated from the reaction mixture were determined to be 431 by ESI-Mass. $^1H$ NMR also support the conversion of cephalosporin C to 7$\alpha$-hydroxycephalosporin C by immobilized enzyme.