Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Vitellogenin mRNA Induction in Male African Clawed Frog Treated with di-2-ethylhexyl Phthalate

Di-2-ethylhexyl phthalate 처리 남아프리카산발톱개구리에서의 vitellogenin 발현

  • 박응로 (국립환경연구원 환경위해성연구과) ;
  • 이철우 (국립환경연구원 환경위해성연구과) ;
  • 류지성 (국립환경연구원 환경위해성연구과) ;
  • 남성숙 (국립환경연구원 환경위해성연구과) ;
  • 전성환 (국립환경연구원 환경위해성연구과) ;
  • 나진균 (국립환경연구원 환경위해성연구과) ;
  • 최덕일 (국립환경연구원 환경위해성연구과) ;
  • 박광식 (국립환경연구원 환경위해성연구과)
  • Published : 2001.12.01
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Abstract

The estrogenic potency of di -2-ethylhexyl phthalate (DEHP) using reverse transcriptase-PCR respouse of liver vitellogenin mRNA in male African clawed frog (Xenopus laevis) was studied. Male frogs were injected with DEHP at dose of 300$\mu\textrm{g}$/kg and 300 mg/kg body weight through the dorsal lymph sac. After 4 days, using suitable pair of RT-PCR primers, vitellogenin mRNA induction in the liver was measured and DEHP showed vitellogenin mRNA induction in only the group treated with 300 mg/kg. Any significant histological abnormalities by the exposure of DEHP was not shown in both testis and liver.

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