Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Cassia tora Ethanol Extract on the Lipid Levels of Serum and Liver in Rats Fed High Cholesterol Diet

결명자 에탄올 추출물이 고콜레스테롤 식이를 섭취한 흰쥐의 체내 지질함량에 미치는 영향

  • 하태열 (한국식품개발연구원 쌀연구팀) ;
  • 조일진 (한국식품개발연구원 쌀연구팀) ;
  • 성기승 (한국식품개발연구원 쌀연구팀) ;
  • 이상효 (한국식품개발연구원 쌀연구팀)
  • Published : 2001.12.01
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Abstract

This study was undertaken to investigate the effect of Cassia tora ethanol extract on the lipid levels in serum and liver of rats fed high cholesterol diet. Experimental rats were divided into the following 4 groups: normal diet group, high cholesterol diet group, high choleslerol-0.25% C. tora ethanol extract group and high cholesterol-0.5% C. tora ethanol extract group. After 4 weeks, rats were sacrificed and analyzed the serum lipid profiles, activities of serum alanine aminotransferase (AST), aspartate aminotransferase (ALT), hepatic glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme (ME). It was also determined the contents of total cholesterol, triglyceride and thiobarbituric acid reactive substances (TBARS) in liver. There was no difference in weight gains between experimental groups. The concentrations of serum total cholesterol, free cholesterol, triglyceride and free fatty acid were tended to be decreased in C. tora groups compared with control group. HDL-cholesterol concentration was significantly decreased in high cholesterol diet group and slightly increased by C. tora ethanol extract feeding. The contents of liver cholesterol and triglyceride were higher in high cholesterol diet group than normal group, but significantly decreased by feeding of C. tora ethanol extract. Supplementation of 0.5% C. tora extract decreased significantly the activities of hepatic G6PDH and ME. Activities of serum AST, ALT and contents of liver TBARS were tended to be increased with high cholesterol diet and reduced by C. tora ethanol extract supplementation but had not significance. These results suggest that C. tora ethanol extract may exert a lipid lowering effect in serum and liver of rats.

결명자의 에탄올 추출물이 고콜레스테롤 식이를 섭취한 횐쥐의 체내 지질함량에 미치는 영향을 알아보기 위하여 정상식이군, 고콜레스테롤 대조군, 고콜레스테롤 식이에 결명자 에탄올 추출물을 각각 0.25%와 0.5%씩 첨가하여 공급하는 실험군으로 나누어 4주간 사육한 후 혈청 및 간장의 지질 함량, glucose-6-phosphate dehydrogenase와 malic enzyme 활성변화, 혈청 AST, ALT활성 및 지질과산화물의 함량의 변화를 조사하였다. 체중증가량과 식이섭취량은 추출물을 공급한 실험군에서 높았으나, 식이효율은 각 군간 차이가 없었다. 결명자 추출물군의 혈청 총콜레스테롤, 유리콜레스테롤, 중성지방 및 유리지방산 함량은 대조군에 비하여 감소하는 경향이었다. 간장중의 총 콜레스테롤과 중성지방의 함량은 고콜레스테롤 식이에 의하여 유의하게 증가하였으나 결명자 추출물을 첨가함으로서 증가된 간장 지질함량은 유의하게 감소하였다. 간장의 glucose-6-phosphate dehydrogenase와 magic enzyme 활성 을 측정한 결과, 결명자 추출물군에서는 대조군에 비하여 활성이 유의하게 감소하였으며, 추 출물의 첨가농도가 높을수록 현저하였다. ALT, AST 활성 역시 결명자 추출물군은 고콜레스테롤 대조군에 비하여 감소하는 경향이었고, 간장 조직중의 지질과산화물 함량도 결명자 추출물을 첨가함에 따라 통계적 유의차는 없었으나 감소하는 경향이었다.

Keywords

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