Cytotoxicity of the ethanol extracts of mulberry leaves, branches and silkworm feces

상엽, 상지 및 잠분 에탄올 추출물의 품종별 세포독성 효과

  • 안미영 (농업과학기술원 잠사곤충부) ;
  • 류강선 (농업과학기술원 잠사곤충부) ;
  • 김익수 (농업과학기술원 잠사곤충부) ;
  • 김선여 (경희대학교 동서의학대학원) ;
  • 이희삼 (농업과학기술원 잠사곤충부)
  • Published : 2001.06.01

Abstract

The cytotoxicity of the ethanol extracts of varietal samples of mulberry leaves and branches and silkworm feces was measured using CT-26 cells originated from murine metastatic colon cancer, using dye uptake assay in order to find potential anticancer agents. Two ethanol extracts (varietal mulberry leaves and mulberry branches) were prepared from 16 varietal mulberries and used as partial extract materials for the activity assay. Among these, the ethanol extracts from Shinkwangppong leaves showed a little anticancer activity, and those from Sugaeppong, Cheongunppong and Gumsulppong branches showed some anticancer activity as well as cytotoxicity. In contrast, ethanol extracts from freeze-dried, the 3rd day of 5th instar feces showed more potent anticancer activity than that of other mulberry leaves, mulberry branches and other 5th silkworm instar larva feces on the basis of high UV absorbance at 665 nm.

Keywords

References

  1. Cancer Chemother. Pharmacol. v.44 Cytotoxicity and neurocytotoxicity of new marine anticancer agents evaluated using in vitro assays Geldof, A. A., S. C. Mastbergen, R. E. Henrar;G. T. Faircloth
  2. Int. J. Indut. Entomol. v.2 Variation of mineal compositions in the regional, varietal and seasonal mulberry leaves Kim, I., H.S.Lee, J.W. Kim, B. K. Yang, M. Y. Ahn, D. H. Kim;K. S. Ryu
  3. Arch. Pharm. Res. v.23 Cytotoxicity and L-Amino Acid Oxidase Activity of Insect Crude Drugs Ahn, M. Y., K. S. Ryu, Y. W. Lee;Y. S. Kim
  4. Ch. Springer-Verlag v.73 Progress in the Chemistry of Organic Natural Products Nomura, T.;T. Fukai(ed by)Herz, W. Kirby, G.W. Moore, R.E. Steglich, W. Tamm
  5. 1999년 한국잠사학회 심포지움 뽕잎함유 생체활성성분의 식품이용전망 이완주
  6. Yonsei Medical Journal v.31 Chlorophyll derivatives (CpD) extracted from silk worm excreta specially cytotoxic to tumor cells in vitro Lee, W. Y., J. H. Park, B. S. Kim, M. J. Han;B. S. Hahn