Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Growth Optimization of Photorhabdus luminescens Isolated from Entomopathogenic Nematode Heterorhabditis bacteriophora

병원성 선충 Heterorhabditis bacteriophora에서 분리된 공생 박테리아 Photorhabdus luminescens의 생장조건

  • Yoo, Sun Kyun (Department of Entomology, Rutgers University New Brunswick) ;
  • Randy Gaugler (Department of Entomology, Rutgers University New Brunswick) ;
  • Christopher W. Brey (Department of Entomology, Rutgers University New Brunswick)
  • Published : 2001.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The yield of infective juveniles of Heterorhabditis bacteriophora (Tf strain) in vitro monoxenic liquid culture was improved significantly as the amount of symbiont biomass, Photorhabdus sp. strain Tf, increased. To investigate the influence of abiotic factors on the growth and biomass production of Photorhabdus sp. strain Tf, triplicate flask cu1tmes were performed. The optinal temperature and medium pH for the growth of Photorhahdus sp. strain Tf were 30$^{\circ}$C and between pH 5.5-7.3, respectively. Aeration also improved greatly growth and yield of biomass of Photorhabdus sp. strain Tf. Photorhabdus sp. strain Tf in batch fermentation showed growth-associated pattem in terms of pigment production, and the pH of culture medium rose steadily until growth stopped dUling the fermentation. Both pigment production and culture pH rise would be useful parameters indicating a reliable growth of Photorhabdus sp. strain Tf.

공생 박테리아 Photorhabdus sp. strain TF 균체량이 증가함에 따라서 병원성 선충 Heterorhabditis bacteriophora의 Infective Juveniles의 생산이 증가되었다. 이 공생 박테리아의 성장 최적 조건은 각각 배양 온도 3$0^{\circ}C$ 그리고 초기 배지 pH 5.5부터 7.3 사이에서 얻어졌다. 통기 상태에서 박테리아 균체의 생산과 성장이 촉진됨이 밝혀졌다. Photorhabdus sp. strain TF 박테리아의 성장 중 생산된 색소와 배양액의 pH의 변화는 박테리아의 성장 정도 나타내어 액체배양에서 병원성 선충 Heterorhabditis bacteriophora의 접종시기로 표시로서 이용할 수 있을 것이다.

Keywords

References

  1. J. Gen. Microbiol. v.121 Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically associated with insect pathogenic nematodes Neoapectana and Heterorhabditis Akhurst, R. J.
  2. J. Gen. Microbiol. v.128 Antibiotic activity of Xenorhabdus spp., bacteria symbiotically associated with insect pathogenic nematodes of the family Heterorhabditidae and Steinernematidae Akhurst, R. J.
  3. FEMS Microbiol. Ecol. v.53 Characterization of primary and secondary forms of Xenorhabdus luminescens strains HM Bleakley, B.;K. N. Nealson
  4. J. Gen. Microbiol. v.134 Biochemical and physiological characterization of colony form variants in Xenorhabdus spp. (Enterobacteriaceae) Boemare, N. E.;R. J. Akhurst
  5. Nematologica v.13 The demonstration of sterols as requirement of growth, development, reproduction pf the DD-136 nematode Dutky, S. R.;W. E. Robbins;J. V. Thompson
  6. Nematodes in Biological control Commercial production and development Friedman, M. J.;R. Gaugler(ed.);H.-K.Kaya(ed.)
  7. Nature v.218 A pheromone influences larval development in the nematode Caenorhadditis elegans Golden, J. W.;D. L. Riddle
  8. J. Invertebr. Pathol. v.75 Pathogenicity, development and reproduction of Heterorhabditis bacteriophora and Steinernema carpocapsae under axenic in vivo conditions Han, R.;R. U. Ehlers
  9. Appl. Environ Microbiol. v.64 In vitro and in vivo characterization of a small-colony variant of the primary form of Photorhabdus luminescens MD Hu, K.;J. M. Webster
  10. FEMS. Microbiol. Lett. v.42 The effect of inophores on the production of extracellular dextransucrase by Leuconostoc mesenteroides Otts, D. R.;D. F. Day
  11. Soil. Biol. Biochem v.12 Growth and luminescens of the symbiotic bacteria associated with terrestrial nematodes, Heterorhabditis bacteriophora. Poiner, G. O.;G. Thomas;M. Haygood;K. H. Nealson
  12. Appl. Environ. Microbiol. v.54 Identification of an anthraquinone pigment and a hydroxystilbene antibiotic from Xenorhabdus luminescens Richardson, W. H.;T. M. Schmidt;K. H. Nealson
  13. Appl. Microbiol. Biotechnol. v.50 Food signal production of Photorhabdus luminescens inducing the recovery of entomopathogenic nematodes Heterorhabditis spp. in liquid culture Strauch, O.;R. U. Ehlers
  14. Appl. Environ. Microbiol. v.55 Bioluminescence of the insect pathogen Xenorhabdus luminescens Schmidt, T. M.;K. Kopecky;K. H. Nealson
  15. Appl. Microbiol. Biotechnol. v.54 Liquid media development for Heterorhabditis bacteriophora: lipid source and concentration Yoo, S. K.;I. Brown;R. Gaugler